PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC

被引：4
|
作者：
Chen, Tao [1 ,2 ]
Wang, Ping [1 ,2 ]
Du, Yuzhi [1 ]
Shen, Yuhu [1 ]
Li, Yulin [1 ]
机构：
[1] Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai Prov, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2012年 / 35卷 / 18期
基金：
中国国家自然科学基金;
关键词：
barley seedlings; Hordeum vulgare; HPLC; HSCCC; lutonarin; saponarin; SPEED COUNTERCURRENT CHROMATOGRAPHY; LIQUID-CHROMATOGRAPHY; HORDEUM-VULGARE; FLAVONOIDS; LEAVES;
D O I：
10.1080/10826076.2011.636471
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (Hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (HSCCC). Separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). A total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. High performance liquid chromatography (HPLC) analysis showed that the purity of each of the two compounds was over 98%. Their chemical structures were confirmed by UV, H-1 NMR, and C-13 NMR data.
引用
收藏
页码：2524 / 2532
页数：9
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