Borate enhances the production of lactulose from lactose by cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus

被引：36
|
作者：
Kim, Yeong-Su [1 ]
Kim, Jung-Eun [1 ]
Oh, Deok-Kun [1 ]
机构：
[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
来源：
BIORESOURCE TECHNOLOGY | 2013年 / 128卷
基金：
新加坡国家研究基金会;
关键词：
Lactulose; Lactose; Borate; Cellobiose; 2-epimerase; Caldicellulosiruptor saccharolyticus; ENZYMATIC-SYNTHESIS; ARABINOSE; FRUCTOSE; B-11;
D O I：
10.1016/j.biortech.2012.10.060
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus was used in the presence of borate to increase the production of lactulose from lactose. Maximum production of lactulose occurred using a 1:1 M ratio of borate-lactose. Under this condition, the enzyme produced 614 g l(-1) lactulose from 700 g l(-1) lactose after incubation at pH 7.5 and 80 degrees C for 3 h, with a conversion yield of 88% and a productivity of 205 g l(-1) h(-1). The yield and productivity of lactulose production obtained in the present study are among the highest achieved through chemical or biological synthesis. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：809 / 812
页数：4
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