Production of Ginsenoside Rd from Ginsenoside Rc by α-L-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus

被引：14

作者：

Shin, Kyung-Chul ^{[1
]}

Lee, Gi-Woong ^{[1
]}

Oh, Deok-Kun ^{[1
]}

机构：

[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 23卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Ginsenoside Rd; ginsenoside Rc; biotransformation; alpha-L-arabinofuranosidase; Caldicellulosiruptor saccharolyticus;

D O I：

10.4014/jmb.1211.11012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ginsenoside Rd was produced from ginsenoside Re using a thermostable recombinant alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus. The optimal reaction conditions for the production of ginsenoside Rd from Re were pH 5.5, 80 degrees C, 227 U enzyme/ml, and 8.0 g/l ginsenoside Re in the presence of 30% (v/v) n-hexane. Under these conditions, the enzyme produced 7.0 g/l ginsenoside Rd after 30 min, with a molar yield of 100% and a productivity of 14 g l(-1) h(-1). The conversion yield and productivity of ginsenoside Rd are the highest reported thus far among enzymatic transformations.

引用

页码：483 / 488

页数：6

共 50 条

[1] Hydrolytic properties of a thermostable α-l-arabinofuranosidase from Caldicellulosiruptor saccharolyticus
Lim, Y. -R.
Yoon, R. -Y.
Seo, E. -S.
Kim, Y. -S.
Park, C. -S.
Oh, D. -K.
JOURNAL OF APPLIED MICROBIOLOGY, 2010, 109 (04) : 1188 - 1197
[2] Characterization of a novel arabinose-tolerant -l-arabinofuranosidase with high ginsenoside Rc to ginsenoside Rd bioconversion productivity
Xie, J.
Zhao, D.
Zhao, L.
Pei, J.
Xiao, W.
Ding, G.
Wang, Z.
Xu, J.
JOURNAL OF APPLIED MICROBIOLOGY, 2016, 120 (03) : 647 - 660
[3] Characterization of a novel arabinose-tolerant α-L-arabinofuranosidase with high ginsenoside Rc to ginsenoside Rd bioconversion productivity
Zhao, L. (lg.zhao@163.com), 1600, (120):
[4] Purification and characterization of an α-l-arabinofuranosidase, α-L-AFase, for hydrolyzed ginsenoside Rc from Bacillus subtilis
Zhu, Ling
Xu, Qingfang
Liu, Weiliang
Xu, Qihe
Zhang, Lifang
Gao, Xiu
Cai, Jian
PROTEIN EXPRESSION AND PURIFICATION, 2024, 217
[5] Compound K Production from Red Ginseng Extract by β-Glycosidase from Sulfolobus solfataricus Supplemented with α-L-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus
Shin, Kyung-Chul
Choi, Hye-Yeon
Seo, Min-Ju
Oh, Deok-Kun
PLOS ONE, 2015, 10 (12):
[6] An L213A variant of β-glycosidase from Sulfolobus solfataricus with increased α-L-arabinofuranosidase activity converts ginsenoside Rc to compound K
Choi, Ji-Hyeon
Shin, Kyung-Chul
Oh, Deok-Kun
PLOS ONE, 2018, 13 (01):
[7] Production of compond K from major protopanaxadiol ginsenosides by combined enzymes, including α-L-arabinofuranosidase and β-galactosidase from Caldicellulosiruptor saccharolyticus and β-glucosidase from Sulfolobus acidocaldarius
Shin, Kyung-Chul
Oh, Hye-Jin
Kim, Baek-Joong
Oh, Deok-Kun
NEW BIOTECHNOLOGY, 2014, 31 : S81 - S81
[8] Two Key Amino Acids Variant of α-l-arabinofuranosidase from Bacillus subtilis Str. 168 with Altered Activity for Selective Conversion Ginsenoside Rc to Rd
Zhang, Ru
Tan, Shi Quan
Zhang, Bian Ling
Guo, Zi Yu
Tian, Liang Yu
Weng, Pei
Luo, Zhi Yong
MOLECULES, 2021, 26 (06):
[9] Bioconversion of ginsenoside Rc into Rd by a novel α-l-arabinofuranosidase, Abf22-3 from Leuconostoc sp 22-3: cloning, expression, and enzyme characterization
Liu, Qing-Mei
Jung, Hae-Min
Cui, Chang-Hao
Sung, Bong-Hyun
Kim, Jin-Kwang
Kim, Song-Gun
Lee, Sung-Taik
Kim, Sun-Chang
Im, Wan-Taek
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2013, 103 (04): : 747 - 754
[10] Molecular cloning, expression, purification, and characterization of Bacillus subtilis hydrolyzed ginsenoside Rc of α-L-arabinofuranosidase in Escherichia coli
Zhu, Ling
Wang, Yuchen
Cai, Jian
ARCHIVES OF MICROBIOLOGY, 2024, 206 (04)

← 1 2 3 4 5 →