Production of Ginsenoside Rd from Ginsenoside Rc by α-L-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus

被引：14
|
作者：
Shin, Kyung-Chul [1 ]
Lee, Gi-Woong [1 ]
Oh, Deok-Kun [1 ]
机构：
[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 23卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
Ginsenoside Rd; ginsenoside Rc; biotransformation; alpha-L-arabinofuranosidase; Caldicellulosiruptor saccharolyticus;
D O I：
10.4014/jmb.1211.11012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Ginsenoside Rd was produced from ginsenoside Re using a thermostable recombinant alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus. The optimal reaction conditions for the production of ginsenoside Rd from Re were pH 5.5, 80 degrees C, 227 U enzyme/ml, and 8.0 g/l ginsenoside Re in the presence of 30% (v/v) n-hexane. Under these conditions, the enzyme produced 7.0 g/l ginsenoside Rd after 30 min, with a molar yield of 100% and a productivity of 14 g l(-1) h(-1). The conversion yield and productivity of ginsenoside Rd are the highest reported thus far among enzymatic transformations.
引用
收藏
页码：483 / 488
页数：6
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