Overcoming Transcription Activator-like Effector (TALE) DNA Binding Domain Sensitivity to Cytosine Methylation

被引：141

作者：

Valton, Julien ^{[1
]}

Dupuy, Aurelie ^{[1
]}

Daboussi, Fayza ^{[1
]}

Thomas, Severine ^{[1
]}

Marechal, Alan ^{[1
]}

Macmaster, Rachel ^{[1
]}

Melliand, Kevin ^{[1
]}

Juillerat, Alexandre ^{[1
]}

Duchateau, Philippe ^{[1
]}

机构：

[1] Cellectis SA, F-75013 Paris, France

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2012年 / 287卷 / 46期

关键词：

GENE; CANCER; RECOGNITION;

D O I：

10.1074/jbc.C112.408864

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Within the past 2 years, transcription activator-like effector (TALE) DNA binding domains have emerged as the new generation of engineerable platform for production of custom DNA binding domains. However, their recently described sensitivity to cytosine methylation represents a major bottleneck for genome engineering applications. Using a combination of biochemical, structural, and cellular approaches, we were able to identify the molecular basis of such sensitivity and propose a simple, drug-free, and universal method to overcome it.

引用

页码：38427 / 38432

页数：6

共 50 条

[41] Targeted Mutagenesis of the Tomato PROCERA Gene Using Transcription Activator-Like Effector Nucleases
Lor, Vai S.
Starker, Colby G.
Voytas, Daniel F.
Weiss, David
Olszewski, Neil E.
PLANT PHYSIOLOGY, 2014, 166 (03) : 1288 - +
[42] Transcription activator-like effector hybrids for conditional control and rewiring of chromosomal transgene expression
Li, Yi
Moore, Richard
Guinn, Michael
Bleris, Leonidas
SCIENTIFIC REPORTS, 2012, 2
[43] Transcription activator-like effector nucleases (TALENs): An efficient tool for plant genome editing
Chaudhary, Kulbhushan
Pratap, Dharmendra
Sharma, Pradeep K.
ENGINEERING IN LIFE SCIENCES, 2016, 16 (04): : 330 - 337
[44] The application of transcription activator-like effector nucleases for genome editing in C-elegans
Yi, Peishan
Li, Wei
Ou, Guangshuo
METHODS, 2014, 68 (03) : 389 - 396
[45] Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)
Kowalko, Johanna E.
Ma, Li
Jeffery, William R.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2016, (112):
[46] Use of transcription activator-like effector for efficient gene modification and transcription in the filamentous fungus Trichoderma reesei
Liu, Pei
Wang, Wei
Wei, Dongzhi
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2017, 44 (09) : 1367 - 1373
[47] Upregulation of the Pluripotency-Associated miRNA 302-367 Cluster Using Engineered Transcription Activator-Like Effector (TALE) Activators
Maeder, Morgan L.
Angstman, James F.
Linder, Sam J.
Reyon, Deepak
Joung, J. Keith
MOLECULAR THERAPY, 2012, 20 : S193 - S193
[48] Transcription activator-like effector protects bacterial endosymbionts from entrapment within fungal hyphae
Richter, Ingrid
Wein, Philipp
Uzum, Zerrin
Stanley, Claire E.
Krabbe, Jana
Molloy, Evelyn M.
Moebius, Nadine
Ferling, Iuliia
Hillmann, Falk
Hertweck, Christian
CURRENT BIOLOGY, 2023, 33 (13) : 2646 - +
[49] Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos
Lei, Yong
Guo, Xiaogang
Deng, Yi
Chen, Yonglong
Zhao, Hui
CELL AND BIOSCIENCE, 2013, 3
[50] Transcription Activator-Like Effector Nuclease-Based Genome Editing for Mucopolysaccharidosis Type I
Osborn, Mark J.
Starker, Colby G.
DeFeo, Anthony P.
Voytas, Daniel F.
Blazar, Bruce R.
Tolar, Jakub
MOLECULAR THERAPY, 2013, 21 : S224 - S224

← 1 2 3 4 5 →