Overcoming Transcription Activator-like Effector (TALE) DNA Binding Domain Sensitivity to Cytosine Methylation

被引：141

作者：

Valton, Julien ^{[1
]}

Dupuy, Aurelie ^{[1
]}

Daboussi, Fayza ^{[1
]}

Thomas, Severine ^{[1
]}

Marechal, Alan ^{[1
]}

Macmaster, Rachel ^{[1
]}

Melliand, Kevin ^{[1
]}

Juillerat, Alexandre ^{[1
]}

Duchateau, Philippe ^{[1
]}

机构：

[1] Cellectis SA, F-75013 Paris, France

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2012年 / 287卷 / 46期

关键词：

GENE; CANCER; RECOGNITION;

D O I：

10.1074/jbc.C112.408864

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Within the past 2 years, transcription activator-like effector (TALE) DNA binding domains have emerged as the new generation of engineerable platform for production of custom DNA binding domains. However, their recently described sensitivity to cytosine methylation represents a major bottleneck for genome engineering applications. Using a combination of biochemical, structural, and cellular approaches, we were able to identify the molecular basis of such sensitivity and propose a simple, drug-free, and universal method to overcome it.

引用

页码：38427 / 38432

页数：6

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