Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain

被引：0
|
作者：
Sharma, Nikhil [1 ]
Sen, Ganes C. [1 ]
机构：
[1] Cleveland Clin, Lerner Res Inst, Dept Inflammat & Immun, 9500 Euclid Ave, Cleveland, OH 44195 USA
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 04期
基金：
美国国家卫生研究院;
关键词：
Gene expression; Model organisms; Molecular biology; Neuroscience;
D O I：
10.1016/j.xpro.2022.101836
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and West-ern blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).1
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页数：10
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