Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain

被引：0
|
作者：
Sharma, Nikhil [1 ]
Sen, Ganes C. [1 ]
机构：
[1] Cleveland Clin, Lerner Res Inst, Dept Inflammat & Immun, 9500 Euclid Ave, Cleveland, OH 44195 USA
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 04期
基金：
美国国家卫生研究院;
关键词：
Gene expression; Model organisms; Molecular biology; Neuroscience;
D O I：
10.1016/j.xpro.2022.101836
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and West-ern blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).1
引用
收藏
页数：10
相关论文
共 50 条
  • [31] Antisense locked nucleic acids targeting agrA inhibit quorum sensing and pathogenesis of community-associated methicillin-resistant Staphylococcus aureus
    Da, F.
    Yao, L.
    Su, Z.
    Hou, Z.
    Li, Z.
    Xue, X.
    Meng, J.
    Luo, X.
    JOURNAL OF APPLIED MICROBIOLOGY, 2017, 122 (01) : 257 - 267
  • [32] Topical gene silencing by iontophoretic delivery of an antisense oligonucleotide-dendrimer nanocomplex: the proof of concept in a skin cancer mouse model
    Venuganti, Venkata Vamsi K.
    Saraswathy, Manju
    Dwivedi, Chandradhar
    Kaushik, Radhey S.
    Perumal, Omathanu P.
    NANOSCALE, 2015, 7 (09) : 3903 - 3914
  • [33] Electroporation-based delivery of cell-penetrating peptide conjugates of peptide nucleic acids for antisense inhibition of intracellular bacteria
    Ma, Sai
    Schroeder, Betsy
    Sun, Chen
    Loufakis, Despina Nelie
    Cao, Zhenning
    Sriranganathan, Nammalwar
    Lu, Chang
    INTEGRATIVE BIOLOGY, 2014, 6 (10) : 973 - 978
  • [34] Direct intracerebral delivery of a miR-33 antisense oligonucelotide into mouse brain increases brain ABCA1 expression
    Jan, Asad
    Karasinska, Joanna M.
    Kang, Martin H.
    de Haan, Willeke
    Ruddle, Piers
    Kaur, Achint
    Connolly, Colum
    Leavitt, Blair R.
    Sorensen, Poul H.
    Hayden, Michael R.
    NEUROSCIENCE LETTERS, 2015, 598 : 66 - 72
  • [35] Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2′-O-methyl RNA, phosphorothioates and small interfering RNA
    Grünweller, A
    Wyszko, E
    Bieber, B
    Jahnel, R
    Erdmann, VA
    Kurreck, J
    NUCLEIC ACIDS RESEARCH, 2003, 31 (12) : 3185 - 3193
  • [36] Quantitation of locked nucleic acid antisense oligonucleotides in mouse tissue using a liquid-liquid extraction LC-MS/MS analytical approach
    Turnpenny, Paul
    Rawal, Jaiessh
    Schardt, Torsten
    Lamoratta, Sandro
    Mueller, Heiko
    Weber, Markus
    Brady, Kevin
    BIOANALYSIS, 2011, 3 (17) : 1911 - 1921
  • [37] Strategies for optimizing lipid-based delivery of nucleic acids to cells for gene delivery and gene silencing: Synthesis, live cell imaging, and transfection efficiency studies
    Ewert, Kai K.
    Majzoub, Ramsey N.
    Chan, Chialing
    Liang, Keng S.
    Safinya, Cyrus R.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
  • [38] QUANTITATIVE ALTERATIONS OF NUCLEIC-ACIDS AND PROTEIN IN THE DEVELOPMENTAL MOUSE-BRAIN OF CONVULSIVE EL STRAIN
    OHNO, K
    YAMAGAMI, S
    MORI, K
    TSUJI, M
    KAWAKITA, Y
    FOLIA PSYCHIATRICA ET NEUROLOGICA JAPONICA, 1980, 34 (03): : 364 - 364
  • [39] Challenges of Assessing Exon 53 Skipping of the Human DMD Transcript with Locked Nucleic Acid-Modified Antisense Oligonucleotides in a Mouse Model for Duchenne Muscular Dystrophy
    Engelbeen, Sarah
    O'Reilly, Daniel
    van de Vijver, Davy
    Verhaart, Ingrid
    van Putten, Maaike
    Hariharan, Vignesh
    Hassler, Matthew
    Khvorova, Anastasia
    Damha, Masad J.
    Aartsma-Rus, Annemieke
    NUCLEIC ACID THERAPEUTICS, 2023, 33 (06) : 348 - 360
  • [40] Tetrahedral Framework Nucleic Acids as an Efficient Nasal-to-Brain Delivery Carrier via Neural Transport Pathways
    Zhang, Geru
    Gao, Yang
    Yao, Lan
    Huang, Liwei
    Yao, Yangxue
    Lin, Yunfeng
    Tian, Taoran
    Cai, Xiaoxiao
    ADVANCED FUNCTIONAL MATERIALS, 2025,
12345