Nucleic acid extraction from Corylus avellana tissues

被引：2

作者：

Bassil, NV ^{[1
]}

Davis, J ^{[1
]}

Azarenko, AN ^{[1
]}

Mehlenbacher, SA ^{[1
]}

机构：

[1] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA

来源：

PROCEEDINGS OF THE FIFTH INTERNATIONAL CONGRESS ON HAZELNUT | 2001年 / 556期

关键词：

DNA; RNA; PCR; RAPD;

D O I：

10.17660/ActaHortic.2001.556.34

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

A protocol for large-scale DNA extraction from young hazelnut leaves and catkins was developed. It is suitable for daily extractions of 200 to 300 samples and requires 2-5 days to complete. About 97% of the DNA samples obtained are amenable to amplification by the polymerase chain reaction. Six methods were compared for RNA isolation. Good quality RNA was successfully isolated from young hazelnut leaves and styles using the RNeasy Plant Kit (Qiagen) with modifications to the manufacturer's protocol. Yet a CTAB (hexadecyltrimethylammonium bromide) method originally used to isolate RNA from pine needles was superior to all five RNeasy Plant Kit-based protocols.

引用

页码：241 / 246

页数：6

共 50 条

[31] QUERCETIN 3-GLUCOSYLGALACTOSIDE FROM POLLEN OF CORYLUS-AVELLANA
STRACK, D
MEURER, B
WRAY, V
GROTJAHN, L
AUSTENFELD, FA
WIERMANN, R
PHYTOCHEMISTRY, 1984, 23 (12) : 2970 - 2971
[32] Phytophagous insects on hazel (Corylus avellana) in Switzerland
Kamke, MC
MITTEILUNGEN DER DEUTSCHEN GESELLSCHAFT FUR ALLGEMEINE UND AGEWANDTE ENTOMOLOGIE, BAND 11, HEFT 1-6, DEZEMBER 1997: ENTOMOLOGISTS CONFERENCE, 1997, 11 (1-6): : 285 - 288
[33] A new genus of Bambusicolaceae (Pleosporales) on Corylus avellana (Fagales) from Italy
Wijesinghe, Subodini Nuwanthika
Wang, Yong
Camporesi, Erio
Wanasinghe, Dhanushka Nadeeshan
Boonmee, Saranyaphat
Hyde, Kevin David
BIODIVERSITY DATA JOURNAL, 2020, 8
[34] CHEMICAL COMPOSITION OF EXTRACT OF Corylus avellana SHELLS
Shataer, D.
Abdulla, R.
Ma, Q. L.
Liu, G. Y.
Aisa, H. A.
CHEMISTRY OF NATURAL COMPOUNDS, 2020, 56 (02) : 338 - 340
[35] Chemical Composition of Extract of Corylus avellana Shells
D. Shataer
R. Abdulla
Q. L. Ma
G. Y. Liu
H. A. Aisa
Chemistry of Natural Compounds, 2020, 56 : 338 - 340
[36] Micropropagation of hazelnut (Corylus avellana L.)
Damiano, C
Catenaro, E
Giovinazzi, J
Frattarelli, A
Caboni, E
PROCEEDINGS OF THE VITH INTERNATIONAL CONGRESS ON HAZELNUT, 2005, (686): : 221 - 226
[37] REDUCTION OF EASTERN FILBERT BLIGHT ON CORYLUS AVELLANA
KOSZTARAB, M
ROANE, MK
DRAKE, CR
PHYTOPATHOLOGY, 1980, 70 (07) : 690 - 690
[38] PHOSPHORUS COMPOUNDS IN ANIMAL TISSUES .1. EXTRACTION AND ESTIMATION OF DESOXYPENTOSE NUCLEIC ACID AND OF PENTOSE NUCLEIC ACID
SCHNEIDER, WC
JOURNAL OF BIOLOGICAL CHEMISTRY, 1945, 161 (01) : 293 - 303
[39] Nucleic acid extraction methods from fixed and paraffin-embedded tissues in cancer diagnostics
Bonin, Serena
Stanta, Giorgio
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2013, 13 (03) : 271 - 282
[40] Pollen surface alkane composition of Corylus avellana X Corylus maxima hybrids
Caramiello, R
Maffei, M
Miaja, ML
Me, G
PROCEEDINGS OF THE FIFTH INTERNATIONAL CONGRESS ON HAZELNUT, 2001, (556): : 545 - 551

← 1 2 3 4 5 →