Nucleic acid extraction from Corylus avellana tissues

被引：2
|
作者：
Bassil, NV [1 ]
Davis, J [1 ]
Azarenko, AN [1 ]
Mehlenbacher, SA [1 ]
机构：
[1] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA
来源：
PROCEEDINGS OF THE FIFTH INTERNATIONAL CONGRESS ON HAZELNUT | 2001年 / 556期
关键词：
DNA; RNA; PCR; RAPD;
D O I：
10.17660/ActaHortic.2001.556.34
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
A protocol for large-scale DNA extraction from young hazelnut leaves and catkins was developed. It is suitable for daily extractions of 200 to 300 samples and requires 2-5 days to complete. About 97% of the DNA samples obtained are amenable to amplification by the polymerase chain reaction. Six methods were compared for RNA isolation. Good quality RNA was successfully isolated from young hazelnut leaves and styles using the RNeasy Plant Kit (Qiagen) with modifications to the manufacturer's protocol. Yet a CTAB (hexadecyltrimethylammonium bromide) method originally used to isolate RNA from pine needles was superior to all five RNeasy Plant Kit-based protocols.
引用
收藏
页码：241 / 246
页数：6
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