Validation of a rapid method for extraction of total RNA applied to Leishmania promastigotes

被引：2
|
作者：
Pérez, JA [1 ]
Aragón, Z [1 ]
Piñero, JE [1 ]
del Castillo, A [1 ]
de Armas, F [1 ]
Valladares, B [1 ]
机构：
[1] Univ La Laguna, Dept Parasitol Ecol & Genet, Fac Farm, Tenerife 38271, Spain
来源：
JOURNAL OF PARASITOLOGY | 1999年 / 85卷 / 04期
关键词：
D O I：
10.2307/3285759
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
This report explains a rapid procedure (approximately 50 min) for the isolation of highly purified total RNA from Leishmania promastigotes based on extraction with acidic phenol. The simplicity of the manipulations required make this method ideal for processing multiple samples; the quality of the RNA obtained is suitable for reverse transcription polymerase chain reaction analysis.
引用
收藏
页码：757 / 758
页数：2
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