Validation of a rapid method for extraction of total RNA applied to Leishmania promastigotes

被引：2
|
作者：
Pérez, JA [1 ]
Aragón, Z [1 ]
Piñero, JE [1 ]
del Castillo, A [1 ]
de Armas, F [1 ]
Valladares, B [1 ]
机构：
[1] Univ La Laguna, Dept Parasitol Ecol & Genet, Fac Farm, Tenerife 38271, Spain
来源：
JOURNAL OF PARASITOLOGY | 1999年 / 85卷 / 04期
关键词：
D O I：
10.2307/3285759
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
This report explains a rapid procedure (approximately 50 min) for the isolation of highly purified total RNA from Leishmania promastigotes based on extraction with acidic phenol. The simplicity of the manipulations required make this method ideal for processing multiple samples; the quality of the RNA obtained is suitable for reverse transcription polymerase chain reaction analysis.
引用
收藏
页码：757 / 758
页数：2
相关论文
共 50 条
  • [21] Efficient oil palm total RNA extraction with a total RNA extraction kit
    Habib, S. H.
    Saud, H. M.
    Kausar, H.
    GENETICS AND MOLECULAR RESEARCH, 2014, 13 (02): : 2359 - 2367
  • [22] A RAPID METHOD FOR THE EXTRACTION AND IDENTIFICATION OF RNA IN PLANT LEAF TISSUE
    MILLIKAN, DF
    PICKETT, EE
    PHYTOCHEMISTRY, 1964, 3 (06) : 667 - 670
  • [23] Optimizing total RNA extraction method for human and mice samples
    Zeng, Yumei
    Tang, Xiaoxue
    Chen, Jinwen
    Kang, Xi
    Bai, Dazhang
    PEERJ COMPUTER SCIENCE, 2024, 12
  • [24] Optimizing total RNA extraction method for human and mice samples
    Zeng, Yumei
    Tang, Xiaoxue
    Chen, Jinwen
    Kang, Xi
    Bai, Dazhang
    PEERJ, 2024, 12
  • [25] An efficient method for total RNA extraction from peanut seeds
    C. Huang
    J. F. Picimbon
    H. Q. Li
    Z. Li
    Q. Liu
    W. Liu
    Russian Journal of Plant Physiology, 2012, 59 : 129 - 133
  • [26] CITATION CLASSIC - RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
    BLIGH, EG
    CURRENT CONTENTS, 1978, (52): : 11 - 11
  • [27] An efficient method for total RNA extraction from peanut seeds
    Huang, C.
    Picimbon, J. F.
    Li, H. Q.
    Li, Z.
    Liu, Q.
    Liu, W.
    RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 2012, 59 (01) : 129 - 133
  • [28] RAPID METHOD FOR EXTRACTION AND QUANTITATION OF TOTAL ANTHOCYANIN OF CRANBERRY FRUIT
    DEUBERT, KH
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1978, 26 (06) : 1452 - 1453
  • [29] Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis
    Ker, Henrique Gama
    de Oliveira Aguiar-Soares, Rodrigo Dian
    Roatt, Bruno Mendes
    Moreira, Nadia das Dores
    Coura-Vital, Wendel
    Carneiro, Claudia Martins
    Teixeira-Carvalho, Andrea
    Martins-Filho, Olindo Assis
    Giunchetti, Rodolfo Cordeiro
    da Silveira-Lemos, Denise
    Reis, Alexandre Barbosa
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2013, 76 (04) : 470 - 476
  • [30] TRANSCRIPTION AND PROCESSING OF BETA-TUBULIN MESSENGER-RNA IN LEISHMANIA-DONOVANI PROMASTIGOTES
    ADHYA, S
    DAS, S
    BHAUMIK, M
    JOURNAL OF BIOSCIENCES, 1990, 15 (04) : 249 - 259
12345