Nakamurella aerolata sp. Nov., Isolated from an Automobile Air Conditioning System

A novel white-colored, aerobic, Gram-stain-positive, rod-shaped bacterium, designated strain DB0629(T) was isolated from a motor car evaporator core collected in South Korea. Strain DB0629(T) grew at 10-35 degrees C, pH 6.0-9.0, and 0-5.0% (w/v) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain DB0629(T) belonged to the genus Nakamurella, with the nearest phylogenetic neighbor being Nakamurella lactea DSM 19367(T) (97.6% sequence similarity). The strain comprised diphosphatidylglycerol and phosphatidylinositol as the main polar lipids; MK-8(H-4) as a sole respiratory quinone; meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan and anteiso-C-15:0, anteiso-C-17:0, iso-C-16:0, iso-C-15:0, and C-16:0 as the major fatty acids. The average nucleotide identity (ANI) and in silico DNA-DNA hybridization values between strain DB0629(T) and N. lactea DSM 19367(T) were 74.9% and 20.8%, respectively, which were below the threshold values of 95% and 70%, respectively. The DNA G + C content was 69.5 mol%. Based on the polyphasic taxonomic data, the novel species Nakamurella aerolata sp. nov. is proposed with the type strain DB0629(T) (= KCTC 72726(T) = NBRC 114624(T)).