Spirosoma carri sp nov., isolated from an automobile air conditioning system

A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0406(T), was isolated from an automobile evaporator core collected in Korea. The cells were non-motile, aerobic and rod-shaped. The strain grew at 15-37 degrees C (optimum, 25 degrees C), at pH 6.0-7.0 (optimum, 6.5) and in the presence of 0-1.5% (w/v) NaCl. Phylogenetically, the strain was related to members of the genus Spirosoma (93.7-90.7% 16S rRNA sequence similarities) and showed the highest sequence similarity of 93.7% to Spirosomapulveris JSH5-14(T). The major fatty acids of the strain were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c),C-16:1 omega 5c and C-16:0. The predominant menaquinone was MK-7. The polar lipid profile revealed the presence of phosphatidylethanolamine, an unidentified aminolipid, unidentified aminophospholipids and unidentified lipids. The DNA G+C content of the strain was 58.7 mol%. Based on phenotypic, genotypic and chemotaxonomic data, strain TX0406(T) represents a novel species in the genus Spirosoma, for which the name Spirosoma carri sp. nov. (=KACC 19013(T) =NBRC 112494(T)) is proposed.