Friedmanniella aerolata sp nov., isolated from air

A novel bacterium, strain 7515T-26(T), was isolated from an air sample collected in Taean region, Republic of Korea. Cells were aerobic, Gram-stain-positive, non-flagellated cocci, growing in the temperature, pH and NaCl ranges of 10-33 degrees C, pH 5.0-9.0 and 0-2 % (w/v). It shared high 16S rRNA gene sequence similarity with Friedmanniella lacustris EL-17A(T) (97.6 %), Friedmanniella lucida FA2(T) (96.9 %) and Friedmanniella luteola FA1(T) (96.9 %), showing high sequence similarities of 96.5-97.6 % with members of the genus Friedmanniella. Phylogenetic trees based on 16S rRNA gene sequences showed that strain 7515T-26(T) and members of the genus Friedmanniella formed a compact cluster separable from other genera. The isolate contained anteiso-C-15 : 0, iso-C-14 : 0 3-OH and iso-C-15 : 0 as the major cellular fatty acids, and MK-9(H-4) as the predominant isoprenoid quinone. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown phospholipids and one unknown lipid, and the DNA G+C content was 73.1 mol%. The peptidoglycan type was A3 gamma. It showed DNA-DNA hybridization values of less than 70 % with F. lacustris EL-17A(T). On the basis of the evidence from this polyphasic study, a novel species, Friedmanniella aerolata sp. nov., is proposed. The type strain is 7515T-26(T) (=KACC 17306(T) =DSM 27139(T)).