Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation

被引：19

作者：

Richardson, Jonathan P. ^{[1
]}

Macmillan, Derek ^{[1
]}

机构：

[1] UCL, Dept Chem, London WC1H 0AJ, England

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2008年 / 6卷 / 21期

基金：

英国惠康基金;

关键词：

D O I：

10.1039/b811501j

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Selective protein cleavage at methionine residues is a useful method for the production of bacterially derived protein fragments containing an N-terminal cysteine residue required for native chemical ligation. Here we describe an optimised procedure for cyanogen bromide-mediated protein cleavage, and ligation of the resulting fragments to afford biologically active proteins.

引用

页码：3977 / 3982

页数：6

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