Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation

被引：19

作者：

Richardson, Jonathan P. ^{[1
]}

Macmillan, Derek ^{[1
]}

机构：

[1] UCL, Dept Chem, London WC1H 0AJ, England

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2008年 / 6卷 / 21期

基金：

英国惠康基金;

关键词：

D O I：

10.1039/b811501j

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Selective protein cleavage at methionine residues is a useful method for the production of bacterially derived protein fragments containing an N-terminal cysteine residue required for native chemical ligation. Here we describe an optimised procedure for cyanogen bromide-mediated protein cleavage, and ligation of the resulting fragments to afford biologically active proteins.

引用

页码：3977 / 3982

页数：6

共 50 条

[31] Synthesis of ω-Conotoxin MVIIA by Native Chemical Ligation
Dai Xiandong
Fan Chongxu
Cao Ying
Liu Shangyi
Jiang Hui
Chen Jisheng
ACTA CHIMICA SINICA, 2010, 68 (07) : 667 - 671
[32] Recent extensions to native chemical ligation for the chemical synthesis of peptides and proteins
Malins, Lara R.
Payne, Richard J.
CURRENT OPINION IN CHEMICAL BIOLOGY, 2014, 22 : 70 - 78
[33] A novel chemical ligation strategy for the chemical synthesis of proteins with native structure
Tchertchian, S
Oplinger, F
Paolini, M
Manganiello, S
Raimondi, S
Depresle, B
Dafflon, N
Gaertner, H
Botti, P
BIOPOLYMERS, 2005, 80 (04) : 496 - 496
[34] Semi-synthesis of (-)-melodinine K via chemical and biological routes
Thi Tran
Walia, Manish
Gardner, Alexander
Teijaro, Christiana
Andrade, Rodrigo
O'Connor, Sarah
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 257
[35] Synthesis of PAF, an antifungal protein from Penicillium chrysogerum by native chemical ligation
Varadi, G.
Batta, G.
Kele, Z.
Toth, G. K.
JOURNAL OF PEPTIDE SCIENCE, 2012, 18 : S68 - S68
[36] Rapid and efficient protein synthesis through expansion of the native chemical ligation concept
Kulkarni, Sameer S.
Sayers, Jessica
Premdjee, Bhavesh
Payne, Richard J.
NATURE REVIEWS CHEMISTRY, 2018, 2 (04) : 1 - 17
[37] Rapid and efficient protein synthesis through expansion of the native chemical ligation concept
Sameer S. Kulkarni
Jessica Sayers
Bhavesh Premdjee
Richard J. Payne
Nature Reviews Chemistry, 2
[38] Chemical Protein Synthesis with the KAHA Ligation
Bode, Jeffrey
BIOPOLYMERS, 2013, 100 (03) : 240 - 241
[39] Chemical Protein Synthesis with the KAHA Ligation
Rohrbacher, Florian
Wucherpfennig, Thomas G.
Bode, Jeffrey W.
PROTEIN LIGATION AND TOTAL SYNTHESIS II, 2015, 363 : 1 - 31
[40] Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation
Janssen, Brian M. G.
van Ommeren, Sven P. F. I.
Merkx, Maarten
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (06): : 12631 - 12647

← 1 2 3 4 5 →