Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection

被引：7

作者：

Leekha, Surbhi ^{[1
]}

Irish, Cole L. ^{[2
]}

Schneider, Susan K. ^{[2
]}

Fernholz, Emily C. ^{[2
]}

Espy, Mark J. ^{[2
]}

Cunningham, Scott A. ^{[2
]}

Patel, Robin ^{[2
]}

Juhn, Young J. ^{[3
]}

Pritt, Bobbi ^{[2
]}

Smith, Thomas F. ^{[2
]}

Sampathkumar, Priya ^{[1
]}

机构：

[1] Mayo Clin, Div Infect Dis, Rochester, MN 55905 USA

[2] Mayo Clin, Div Clin Microbiol, Rochester, MN 55905 USA

[3] Mayo Clin, Dept Pediat & Adolescent Med, Rochester, MN 55905 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2013年 / 75卷 / 02期

关键词：

Multiplex PCR; Respiratory virus; Nasal swab; Throat swab; Group A streptococcus; VIRUSES; PCR; SYMPTOMS; CHILDREN; RHINOVIRUS; CULTURE; ILLNESS; SYSTEM; SWABS;

D O I：

10.1016/j.diagmicrobio.2012.10.016

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We evaluated a commercial multiplex polymerase chain reaction (PCR) assay in a cross-sectional study among 81 adult and pediatric outpatients-40 cases with upper respiratory infection symptoms and 41 asymptomatic controls from February to April 2008. Two specimens (throat swab and nasal swab) from each participant were tested using the EraGen MultiCode-PLx Respiratory Virus Panel that detects 17 viral targets. Throat swabs were also tested for Group A Streptococcus (GAS) by PCR. Respiratory viruses were detected in 22/40 (55%) cases and in 3/41 (7%) controls (P < 0.001). GAS was detected in 10 (25%) cases; GAS and respiratory virus co-infection was found in 4 (10%). Agreement between nasal and throat swabs for viral detection was 69% in cases and 95% in controls. Of 22 cases with a detectable virus, 12 (54%) were picked up by only 1 (throat or nasal) specimen, and the detection rate was increased by combining results of nasal and throat swab testing. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：169 / 173

页数：5

共 50 条

[21] Evaluation of the Accuracy of a Polymerase Chain Reaction-Based - Based Assay for Polymerase Epsilon Mutation Detection in Endometrial Carcinoma
Chen, Tingting
Li, Yang
Li, Xiao
Zhang, Yanyun
Lv, Weiguo
Xie, Xing
Zhang, Xiaofei
Wang, Xinyu
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2024, 148 (08) : 945 - 951
[22] Polymerase chain reaction-based detection of lymphatic filariasis
Fischer, P
Boakye, D
Hamburger, J
MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2003, 192 (01) : 3 - 7
[23] Polymerase chain reaction-based detection of bacteria in semen
Jarvi, K
Dumitru, I
Lacroix, JM
Heritz, D
Jain, A
Mittelman, MW
FERTILITY AND STERILITY, 1996, 66 (03) : 463 - 467
[24] Polymerase chain reaction-based detection of lymphatic filariasis
Peter Fischer
Daniel Boakye
Joseph Hamburger
Medical Microbiology and Immunology, 2003, 192 : 3 - 7
[25] Polymerase Chain Reaction-Based Detection Method Is Suitable for Dengue Infection During Epidemics
Iqbal, Kashif
Hashmi, Abu Saeed
Idrees, Muhammad
Shahzad, Sadeem
Fatima, Zareen
VIRAL IMMUNOLOGY, 2017, 30 (10) : 734 - 736
[26] Utility of an anchored multiplex polymerase chain reaction-based fusion assay for diagnosis of soft tissue tumors in cytology
Helland, T. Leif
Fisch, Adam S.
Chebib, Ivan
CANCER CYTOPATHOLOGY, 2024, 132 (09) : 580 - 587
[27] Multiplex polymerase chain reaction-based reverse line blot hybridization assay to detect common genital pathogens
Wang, H.
Kong, F.
Wang, B.
Mckechnie, M. L.
Gilbert, G. L.
INTERNATIONAL JOURNAL OF STD & AIDS, 2010, 21 (05) : 320 - 325
[28] Detection of Human Bocavirus in Children with Upper Respiratory Tract Infection by Polymerase Chain Reaction
Eyigor, Hulya
Osma, Ustun
Eyigor, Mete
Yilmaz, Mustafa Deniz
Gultekin, Berna
Telli, Murat
Ozturan, Atakan
Gultekin, Meral
CLINICAL LABORATORY, 2013, 59 (1-2) : 139 - 142
[29] Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients
Appak, Ozguer
Duman, Murat
Belet, Nursen
Sayiner, Ayca Arzu
JOURNAL OF MEDICAL VIROLOGY, 2019, 91 (05) : 731 - 737
[30] Development of a polymerase chain reaction-based assay for the detection of Alternaria fungal contamination in food products
Zur, G
Hallerman, EM
Sharf, R
Kashi, Y
JOURNAL OF FOOD PROTECTION, 1999, 62 (10) : 1191 - 1197

← 1 2 3 4 5 →