A simple and rapid protocol for preparation of crude drug DNA suitable for PCR

被引：0
|
作者：
Mizukami, H [1 ]
Okabe, Y [1 ]
机构：
[1] Nagoya City Univ, Fac Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1999年 / 22卷 / 07期
关键词：
DNA purification protocol; molecular authentication; herbal crude drug;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A protocol for rapid purification of DNA from a variety of herbal crude drugs, starting from samples as small as 1 mg, has been developed. Neither phenol extraction nor alcohol precipitation is involved in the procedure. Total nucleic acids are first extracted with the lysis buffer containing SDS. After CHCl3 extraction, DNA is bound to glass fiber fleece in the presence of guanidine thiocyanate, while unbound impurities are washed away. The entire procedure can be carried out in 1.5-ml microtubes. The purified DNA obtained serves as a template for PCR.
引用
收藏
页码：765 / 766
页数：2
相关论文
共 50 条
  • [41] A protocol for rapid DNA extraction from Arabidopsis thaliana for PCR analysis
    Kasajima, I
    Ide, Y
    Ohkama-Ohtsu, N
    Hayashi, H
    Yoneyama, T
    Fujiwara, T
    PLANT MOLECULAR BIOLOGY REPORTER, 2004, 22 (01) : 49 - 52
  • [42] Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100
    Kwon HwangBo
    Su Hyun Son
    Jong Suk Lee
    Sung Ran Min
    Suk Min Ko
    Jang R. Liu
    Dongsu Choi
    Won Joong Jeong
    Plant Biotechnology Reports, 2010, 4 : 49 - 52
  • [43] Rapid, simple and potentially universal method for DNA extraction from Opuntia spp. fresh cladode tissues suitable for PCR amplification
    Joana Raimundo
    Carlos Manuel Gaspar Reis
    Maria Margarida Ribeiro
    Molecular Biology Reports, 2018, 45 : 1405 - 1412
  • [44] Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100
    HwangBo, Kwon
    Son, Su Hyun
    Lee, Jong Suk
    Min, Sung Ran
    Ko, Suk Min
    Liu, Jang R.
    Choi, Dongsu
    Jeong, Won Joong
    PLANT BIOTECHNOLOGY REPORTS, 2010, 4 (01) : 49 - 52
  • [45] Rapid, simple and potentially universal method for DNA extraction from Opuntia spp. fresh cladode tissues suitable for PCR amplification
    Raimundo, Joana
    Gaspar Reis, Carlos Manuel
    Ribeiro, Maria Margarida
    MOLECULAR BIOLOGY REPORTS, 2018, 45 (05) : 1405 - 1412
  • [46] Ammonium acetate protocol for the preparation of plasmid DNA suitable for mammalian cell transfections
    SaporitoIrwin, SM
    Geist, RT
    Gutmann, DH
    BIOTECHNIQUES, 1997, 23 (03) : 424 - 427
  • [47] FOLT PCR - A SIMPLE PCR PROTOCOL FOR AMPLIFYING DNA DIRECTLY FROM WHOLE-BLOOD
    PANACCIO, M
    GEORGESZ, M
    LEW, AM
    BIOTECHNIQUES, 1993, 14 (02) : 238 - +
  • [48] A Simple and Cost-Effective DNA Preparation Method Suitable for High-Throughput PCR Quantification of Hepatitis B Virus Genomes
    Jo, Eunji
    Yang, Jaewon
    Koenig, Alexander
    Yoon, Seung Kew
    Windisch, Marc P.
    VIRUSES-BASEL, 2020, 12 (09):
  • [49] A RAPID AND SIMPLE PROCEDURE FOR THE PREPARATION OF HOMOGENEOUSLY LABELED DNA PROBES
    CULLEN, BR
    GENE, 1986, 43 (03) : 305 - 310
  • [50] A RAPID AND SIMPLE METHOD FOR THE PREPARATION OF YEAST MITOCHONDRIAL-DNA
    QUEROL, A
    BARRIO, E
    NUCLEIC ACIDS RESEARCH, 1990, 18 (06) : 1657 - 1657
12345