A simple and rapid protocol for preparation of crude drug DNA suitable for PCR

被引：0
|
作者：
Mizukami, H [1 ]
Okabe, Y [1 ]
机构：
[1] Nagoya City Univ, Fac Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1999年 / 22卷 / 07期
关键词：
DNA purification protocol; molecular authentication; herbal crude drug;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A protocol for rapid purification of DNA from a variety of herbal crude drugs, starting from samples as small as 1 mg, has been developed. Neither phenol extraction nor alcohol precipitation is involved in the procedure. Total nucleic acids are first extracted with the lysis buffer containing SDS. After CHCl3 extraction, DNA is bound to glass fiber fleece in the presence of guanidine thiocyanate, while unbound impurities are washed away. The entire procedure can be carried out in 1.5-ml microtubes. The purified DNA obtained serves as a template for PCR.
引用
收藏
页码：765 / 766
页数：2
相关论文
共 50 条
  • [11] AN EFFICIENT AND RAPID PROTOCOL FOR PLANT NUCLEAR DNA PREPARATION SUITABLE FOR NEXT GENERATION SEQUENCING METHODS
    Carrier, Gregory
    Santoni, Sylvain
    Rodier-Goud, Marguerite
    Canaguier, Aurelie
    de Kochko, Alexandre
    Dubreuil-Tranchant, Christine
    This, Patrice
    Boursiquot, Jean-Michel
    Le Cunff, Loic
    AMERICAN JOURNAL OF BOTANY, 2011, 98 (01) : E13 - E15
  • [12] A SIMPLE AND RAPID DNA MICROEXTRACTION METHOD FOR PLANT, ANIMAL, AND INSECT SUITABLE FOR RAPD AND OTHER PCR ANALYSES
    CHEUNG, WY
    HUBERT, N
    LANDRY, BS
    PCR-METHODS AND APPLICATIONS, 1993, 3 (01): : 69 - 70
  • [13] A simplified universal genomic DNA extraction protocol suitable for PCR
    Wang, T. Y.
    Wang, L.
    Zhang, J. H.
    Dong, W. H.
    GENETICS AND MOLECULAR RESEARCH, 2011, 10 (01) : 519 - 525
  • [14] Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR
    Izumitsu, Kosuke
    Hatoh, Kanako
    Sumita, Takuya
    Kitade, Yuki
    Morita, Atsushi
    Gafur, Abdul
    Ohta, Akira
    Kawai, Masataka
    Yamanaka, Takashi
    Neda, Hitoshi
    Ota, Yuko
    Tanaka, Chihiro
    MYCOSCIENCE, 2012, 53 (05) : 396 - 401
  • [15] A rapid and cheap protocol for preparation of PCR templates in peanut
    Wang, Chuan Tang
    Wang, Xiu Zhen
    Tang, Yue Yi
    Zhang, Jian Cheng
    Yu, Shan Lin
    Xu, Jian Zhi
    Bao, Zhen Min
    ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2009, 12 (02):
  • [16] A RAPID PROTOCOL FOR THE PURIFICATION OF PLASMID DNA-TEMPLATE SUITABLE FOR DNA SEQUENCING
    MAJUMDAR, MK
    MCANDREWSHILL, M
    WILLIAMS, DA
    ANALYTICAL BIOCHEMISTRY, 1993, 208 (02) : 403 - 404
  • [17] A rapid DNA minipreparation method suitable for AFLP and other PCR applications
    Chen, DH
    Ronald, PC
    PLANT MOLECULAR BIOLOGY REPORTER, 1999, 17 (01) : 53 - 57
  • [18] RAPID ISOLATION OF DNA FROM FOSSIL AND MUSEUM SPECIMENS SUITABLE FOR PCR
    CANO, RJ
    POINAR, HN
    BIOTECHNIQUES, 1993, 15 (03) : 432 - &
  • [19] A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications
    D.-H. Chen
    P.C. Ronald
    Plant Molecular Biology Reporter, 1999, 17 : 53 - 57
  • [20] Rapid isolation of fungal genomic DNA suitable for long distance PCR
    De Maeseneire, S. L.
    Van Bogaert, I. N.
    Dauvrin, T.
    Soetaert, W. K.
    Vandamme, E. J.
    BIOTECHNOLOGY LETTERS, 2007, 29 (12) : 1845 - 1855
12345