A simple and rapid protocol for preparation of crude drug DNA suitable for PCR

被引：0
|
作者：
Mizukami, H [1 ]
Okabe, Y [1 ]
机构：
[1] Nagoya City Univ, Fac Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1999年 / 22卷 / 07期
关键词：
DNA purification protocol; molecular authentication; herbal crude drug;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A protocol for rapid purification of DNA from a variety of herbal crude drugs, starting from samples as small as 1 mg, has been developed. Neither phenol extraction nor alcohol precipitation is involved in the procedure. Total nucleic acids are first extracted with the lysis buffer containing SDS. After CHCl3 extraction, DNA is bound to glass fiber fleece in the presence of guanidine thiocyanate, while unbound impurities are washed away. The entire procedure can be carried out in 1.5-ml microtubes. The purified DNA obtained serves as a template for PCR.
引用
收藏
页码：765 / 766
页数：2
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