Enzyme Based Amperometric Biosensor for Adenine Determination

被引：6
|
作者：
Boka, Beata [1 ]
Adanyi, Nora [2 ]
Virag, Diana [1 ]
Frebort, Ivo [3 ]
Kiss, Attila [1 ]
机构：
[1] Eszterhazy Karoly Coll, EGERFOOD Reg Knowledge Ctr, H-3300 Eger, Hungary
[2] Cent Environm & Food Sci Res Inst, H-1022 Budapest, Hungary
[3] Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, CZ-78371 Olomouc, Czech Republic
来源：
ELECTROANALYSIS | 2013年 / 25卷 / 01期
关键词：
Biosensors; Electrochemistry; Enzymes; Adenine; Adenine deaminase; CAPILLARY-ZONE-ELECTROPHORESIS; PERFORMANCE LIQUID-CHROMATOGRAPHY; GUANINE; ELECTRODE; PURINE; NUCLEOSIDES; OXIDATION; ADENOSINE; ASSAY; BEER;
D O I：
10.1002/elan.201200387
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An enzyme-based amperometric biosensor was developed for the rapid determination of adenine in biological and food samples. Recombinant adenine deaminase (EC 3.5.4.2) from Schizosaccharomyces pombe was co-immobilized on graphite electrode with xanthine oxidase (EC 1.17.3.2), peroxidase (EC 1.11.1.7) and an Os mediator. Optimal working conditions were pH 7.0 and -150 mV vs. Ag/AgCl. The novel method was characterized by a detection limit of 1 mu M and a linear measuring range of 0.0050.1 mM. Different beer and tea samples were analyzed by the newly elaborated adenine biosensor. The results obtained correlate with the adenine content measured by the reference HPLC method.
引用
收藏
页码：237 / 242
页数：6
相关论文
共 50 条
  • [31] A reagentless biosensor for the amperometric determination of NADH
    Cosnier, S
    Decout, JL
    Fontecave, M
    Frier, C
    Innocent, C
    [J]. ELECTROANALYSIS, 1998, 10 (08) : 521 - 525
  • [32] An amperometric biosensor for the determination of organophosphorus pesticides
    Telefoncu, A
    [J]. TURKISH JOURNAL OF CHEMISTRY, 1996, 20 (03): : 253 - 257
  • [33] Amperometric inhibition biosensor for the determination of cyanide
    Ghanavati, Mahdi
    Azad, Reza Roosta
    Mousavi, Seyyed Abbas
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2014, 190 : 858 - 864
  • [34] Amperometric Acetylcholinesterase Biosensor for Physostigmine Determination
    Davletshina, R. R.
    Ivanov, A. N.
    Evtugyn, G. A.
    [J]. UCHENYE ZAPISKI KAZANSKOGO UNIVERSITETA-SERIYA ESTESTVENNYE NAUKI, 2018, 160 (01): : 5 - 16
  • [35] Mediated Amperometric Biosensor for the Determination of Ammonium
    Abd Rahman, Samsulida
    Abdullah, Jaafar
    Sidek, Hamidah
    Azmi, Nur Ellina
    [J]. ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY, 2012, 4 (03): : 262 - 276
  • [36] Development of a sol-gel based amperometric biosensor for the determination of phenolics
    Kane, SA
    Iwuoba, EI
    Smyth, MR
    [J]. ANALYST, 1998, 123 (10) : 2001 - 2006
  • [37] AMPEROMETRIC BIOSENSOR BASED ON ENZYME IMMOBILIZATION WITH POST PROCESS FOR MEDICAL AND MULTIPLE APPLICATIONS
    Gessei, Tomoko
    Arakawa, Takahiro
    Kudo, Hiroyuki
    Saito, Hirokazu
    Mitsubayashi, Kohji
    [J]. ANALYTICAL LETTERS, 2014, 47 (08) : 1361 - 1374
  • [38] An amperometric enzyme biosensor based on in situ electrosynthesized core-shell nanoparticles
    Li, Nian-Feng
    Lei, Ting
    Ouyang, Chun
    He, Yue-Hui
    Liu, Yong
    [J]. SYNTHETIC METALS, 2009, 159 (15-16) : 1608 - 1611
  • [39] A novel amperometric enzyme inhibition biosensor based on xanthine oxidase immobilised onto glassy carbon electrodes for bisphenol A determination
    Ben Messaoud, Najib
    Ghica, Mariana Emilia
    Dridi, Cherif
    Ben Ali, Mounir
    Brett, Christopher M. A.
    [J]. TALANTA, 2018, 184 : 388 - 393
  • [40] A novel zinc finger protein–based amperometric biosensor for miRNA determination
    Eloy Povedano
    Víctor Ruiz-Valdepeñas Montiel
    María Gamella
    Verónica Serafín
    María Pedrero
    Ludmila Moranova
    Martin Bartosik
    Juan José Montoya
    Paloma Yáñez-Sedeño
    Susana Campuzano
    Jose M. Pingarrón
    [J]. Analytical and Bioanalytical Chemistry, 2020, 412 : 5031 - 5041
12345