A biotin-streptavidin signal amplification strategy for a highly sensitive chemiluminescent immunoassay for chicken interferon-γ

被引:10
|
作者
Yang, Zhanjun [1 ]
Luo, Shufen [1 ]
Dai, Hua [1 ]
Li, Juan [1 ]
Jiao, Xin'an [2 ]
Hua, Xiaoya [1 ]
机构
[1] Yangzhou Univ, Coll Chem & Chem Engn, Sch Med, Yangzhou 225002, Peoples R China
[2] Yangzhou Univ, Jiangsu Key Lab Zoonosis, Yangzhou 225002, Peoples R China
来源
RSC ADVANCES | 2013年 / 3卷 / 45期
基金
中国国家自然科学基金;
关键词
RESOLUTION STRATEGY; ENZYME-IMMUNOASSAY; CANCER BIOMARKER; INFECTION; MITOGEN; ANTIBODIES; RESPONSES; ANTIGEN; SYSTEM; ALPHA;
D O I
10.1039/c3ra44325f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The first example of a biotin-streptavidin signal amplification strategy was developed for a highly sensitive chemiluminescent immunoassay for chicken interferon-gamma at sub-picogram per millilitre level. This approach displays a 415-fold increase in the detection limit in comparison with conventional methods.
引用
收藏
页码:22868 / 22871
页数:4
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