A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents

被引：0

作者：

Guillemard, V

Landry, N

Pagé, M ^{[1
]}

机构：

[1] Univ Laval, Dept Med Biol, Fac Med, St Foy, PQ G1K 7P4, Canada

[2] BCM Dev Inc, Quebec City, PQ G1K 4C5, Canada

来源：

ONCOLOGY REPORTS | 1999年 / 6卷 / 06期

关键词：

paclitaxel; taxanes; radioimmunoassay; culture broths;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have developed a rapid and sensitive radioimmunoassay for the measurement of paclitaxel and related taxanes in biological specimens using commercially available reagents. The presence of paclitaxel competitively inhibited the binding of radioactive paclitaxel to polyclonal anti-taxane antibody or anti-taxane monoclonal antibody. When using polyclonal antibody, this method detected paclitaxel in concentrations as low as 0.87 nM and was also effective in 20% methanol. This radioimmunoassay is useful for the measurement of paclitaxel and taxanes in biological specimens like culture medium of paclitaxel-producing microorganisms and may be useful for the measurement of paclitaxel and related compounds in other potential natural sources.

引用

页码：1257 / 1259

页数：3

共 50 条

[41] FREE-THYROXINE DETERMINATION - DIRECT RADIOIMMUNOASSAY OF SERUM DIALYSATES USING A COMMERCIALLY AVAILABLE ANTISERUM AND A POLYETHYLENE GLYCOL SEPARATION
ELSON, MK
SHAFER, RB
CLINICAL CHEMISTRY, 1978, 24 (06) : 1040 - 1040
[42] COMPARISON OF METHODS FOR THE IMMUNOLOGICAL IDENTIFICATION OF NEISSERIA-GONORRHOEAE IN CLINICAL SPECIMENS USING COMMERCIALLY-OBTAINED REAGENTS
FREUNDLICH, LF
ROSENTHAL, SL
HOCHBERG, FP
TROGELE, MR
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1982, 77 (04) : 456 - 458
[43] PLASMA PROGESTERONE ASSAY, USING 2 COMMERCIALLY-AVAILABLE ENZYME-IMMUNOASSAY KITS - COMPARISON WITH A SPECIFIC RADIOIMMUNOASSAY
NAHOUL, K
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1989, 33 (4B): : 837 - 840
[44] Urinary VMA, HVA and 5-HIAA measurement on HPLC with electrochemical detection - A 3-way method comparison study using commercially available reagents
Tan, C. H. C.
Chiong, S. P.
Tan, S. Y.
Leo, S. Y.
Sim, H. T.
Yeo, C. P.
CLINICA CHIMICA ACTA, 2022, 530 : S274 - S274
[45] Detection of high-risk human papillomavirus infection in tonsillar specimens using 2 commercially available assays
Cockerill, Cara C.
Orvidas, Laura J.
Moore, Eric J.
Binnicker, Matthew J.
Duresko, Brian J.
Espy, Mark J.
Cockerill, Franklin R.
Tombers, Nicole M.
Pritt, Bobbi S.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2016, 86 (04) : 365 - 368
[46] Measurement of active shoulder motion using the Kinect, a commercially available infrared position detection system
Matsen, Frederick A., III
Lauder, Alexander
Rector, Kyle
Keeling, Peyton
Cherones, Arien L.
JOURNAL OF SHOULDER AND ELBOW SURGERY, 2016, 25 (02) : 216 - 223
[47] Enzymatic diagnostic test for Muscle-Eye-Brain type congenital muscular dystrophy using commercially available reagents
Zhang, WL
Vajsar, J
Cao, PJ
Breningstall, G
Diesen, C
Dobyns, W
Herrmann, R
Lehesjoki, AE
Steinbrecher, A
Talim, B
Toda, T
Topaloglu, H
Voit, T
Schachter, HY
CLINICAL BIOCHEMISTRY, 2003, 36 (05) : 339 - 344
[48] Validation of radioimmunoassay for salmon insulin-like growth factor-1 using commercially available assay components.
Shimizu, M
Swanson, P
Dickhoff, WW
AMERICAN ZOOLOGIST, 1999, 39 (05): : 29A - 29A
[49] Cobalt-catalyzed synthesis of tertiary azides from α,α-disubstituted olefins under mild conditions using commercially available reagents
Gaspar, Boris
Waser, Jerome
Carreira, Erick M.
SYNTHESIS-STUTTGART, 2007, (24): : 3839 - 3845
[50] Performance Evaluation of Two Methods Using Commercially Available Reagents for PCR-Based Detection of FMR1 Mutation
Juusola, Jane S.
Anderson, Paula
Sabato, Fernanda
Wilkinson, David S.
Pandya, Arti
Ferreira-Gonzalez, Andrea
JOURNAL OF MOLECULAR DIAGNOSTICS, 2012, 14 (05): : 476 - 486

← 1 2 3 4 5 →