A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents

被引：0

作者：

Guillemard, V

Landry, N

Pagé, M ^{[1
]}

机构：

[1] Univ Laval, Dept Med Biol, Fac Med, St Foy, PQ G1K 7P4, Canada

[2] BCM Dev Inc, Quebec City, PQ G1K 4C5, Canada

来源：

ONCOLOGY REPORTS | 1999年 / 6卷 / 06期

关键词：

paclitaxel; taxanes; radioimmunoassay; culture broths;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have developed a rapid and sensitive radioimmunoassay for the measurement of paclitaxel and related taxanes in biological specimens using commercially available reagents. The presence of paclitaxel competitively inhibited the binding of radioactive paclitaxel to polyclonal anti-taxane antibody or anti-taxane monoclonal antibody. When using polyclonal antibody, this method detected paclitaxel in concentrations as low as 0.87 nM and was also effective in 20% methanol. This radioimmunoassay is useful for the measurement of paclitaxel and taxanes in biological specimens like culture medium of paclitaxel-producing microorganisms and may be useful for the measurement of paclitaxel and related compounds in other potential natural sources.

引用

页码：1257 / 1259

页数：3

共 50 条

[31] An Investigation of Measurement Techniques for Small Field Dosimetry Using Commercially Available Detectors
Lim, S.
Tang, G.
LoSasso, T.
MEDICAL PHYSICS, 2013, 40 (06)
[32] A Saccharide Chemosensor Array Developed Based on an Indicator Displacement Assay Using a Combination of Commercially Available Reagents
Sasaki, Yui
Zhang, Zhoujie
Minami, Tsuyoshi
FRONTIERS IN CHEMISTRY, 2019, 7
[33] Scalable efficient expansion of mesenchymal stem cells in xeno free media using commercially available reagents
Neil H Riordan
Marialaura Madrigal
Jason Reneau
Kathya de Cupeiro
Natalia Jiménez
Sergio Ruiz
Nelsy Sanchez
Thomas E Ichim
Francisco Silva
Amit N Patel
Journal of Translational Medicine, 13
[34] A standardized comparison of commercially available prion decontamination reagents using the Standard Steel-Binding Assay
Edgeworth, Julie Ann
Sicilia, Anita
Linehan, Jackie
Brandner, Sebastian
Jackson, Graham S.
Collinge, John
JOURNAL OF GENERAL VIROLOGY, 2011, 92 : 718 - 726
[35] Scalable efficient expansion of mesenchymal stem cells in xeno free media using commercially available reagents
Riordan, Neil H.
Madrigal, Marialaura
Reneau, Jason
de Cupeiro, Kathya
Jimenez, Natalia
Ruiz, Sergio
Sanchez, Nelsy
Ichim, Thomas E.
Silva, Francisco
Patel, Amit N.
JOURNAL OF TRANSLATIONAL MEDICINE, 2015, 13
[36] A NEW RADIOIMMUNOASSAY USING A COMMERCIALLY AVAILABLE SOLID SUPPORT FOR THE DETECTION OF IGE ANTIBODIES AGAINST MUSCLE-RELAXANTS
GUILLOUX, L
RICARDBLUM, S
VILLE, G
MOTIN, J
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 90 (02) : 153 - 159
[37] How to: Measuring blood cytokines in biological psychiatry using commercially available multiplex immunoassays
Belzeaux, Raoul
Lefebvre, Marie-Noelle
Lazzari, Anne
Le Carpentier, Tifenn
Consoloni, Julia-Lou
Zendjidjian, Xavier
Abbar, Mocrane
Courtet, Philippe
Naudin, Jean
Boucraut, Jose
Gressens, Pierre
Glaichenhaus, Nicolas
Ibrahim, El Cherif
PSYCHONEUROENDOCRINOLOGY, 2017, 75 : 72 - 82
[38] Comparison of four commercially available activated partial thromboplastin time reagents using a semi-automated coagulometer
Shetty, S
Ghosh, K
Mohanty, D
BLOOD COAGULATION & FIBRINOLYSIS, 2003, 14 (05) : 493 - 497
[39] One-step synthesis of 4,5-disubstituted pyrimidines using commercially available and inexpensive reagents
Baran, Phil S.
Shenvi, Ryan A.
Nguyen, Steven A.
HETEROCYCLES, 2006, 70 : 581 - +
[40] Gene transfer into primitive human hematopoietic progenitor cells using commercially available lipid transfection reagents.
Musk, P
Lawman, MJP
Burges, AR
Lawman, PD
BLOOD, 1995, 86 (10) : 4002 - 4002

← 1 2 3 4 5 →