Pretreatment of whole blood using hydrogen peroxide and UV irradiation. Design of the advanced oxidation process

被引：8
|
作者：
Bragg, Stefanie A. [1 ]
Armstrong, Kristie C. [1 ]
Xue, Zi-Ling [1 ]
机构：
[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA
来源：
TALANTA | 2012年 / 97卷
基金：
美国国家卫生研究院;
关键词：
Blood; Pretreatment; Advanced oxidation process; Metal analysis; Hydrogen peroxide; ATOMIC-ABSORPTION-SPECTROMETRY; WASTE-WATER TREATMENT; SAMPLE PRETREATMENT; STRIPPING VOLTAMMETRY; QUANTITATIVE-ANALYSIS; BIOLOGICAL SAMPLES; HEAVY-METALS; TRACE-METALS; FENTON; DEGRADATION;
D O I：
10.1016/j.talanta.2012.04.004
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new process to pretreat blood samples has been developed. This process combines the Advanced Oxidation Process (AOP) treatment (using H2O2 and UV irradiation) with acid deactivation of the enzyme catalase in blood. A four-cell reactor has been designed and built in house. The effect of pH on the AOP process has been investigated. The kinetics of the pretreatment process shows that at high C-H2O2,C-t=0, the reaction is zeroth order with respect to CH2O2 and first order with respect to C-blood. The rate limiting process is photon flux from the UV lamp. Degradation of whole blood has been compared with that of pure hemoglobin samples. The AOP pretreatment of the blood samples has led to the subsequent determination of chromium and zinc concentrations in the samples using electrochemical methods. (C) 2012 Elsevier B.V. All tights reserved.
引用
收藏
页码：118 / 123
页数：6
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