Single-molecule detection of coumarin-120

被引：6
|
作者：
Brand, L [1 ]
Eggeling, C [1 ]
Seidel, CAM [1 ]
机构：
[1] MAX PLANCK INST BIOPHYS CHEM,D-37077 GOTTINGEN,GERMANY
来源：
NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 5-6期
关键词：
D O I：
10.1080/07328319708002915
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resulting in nucleobase-specific fluorescence lifetimes. This suggests applications in current projects for ultrasenstive DNA characterization.
引用
收藏
页码：551 / 556
页数：6
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