Single-molecule detection of coumarin-120

被引：6
|
作者：
Brand, L [1 ]
Eggeling, C [1 ]
Seidel, CAM [1 ]
机构：
[1] MAX PLANCK INST BIOPHYS CHEM,D-37077 GOTTINGEN,GERMANY
来源：
NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 5-6期
关键词：
D O I：
10.1080/07328319708002915
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resulting in nucleobase-specific fluorescence lifetimes. This suggests applications in current projects for ultrasenstive DNA characterization.
引用
收藏
页码：551 / 556
页数：6
相关论文
共 50 条
  • [11] SINGLE-MOLECULE DETECTION Focusing on the objective
    Ebenstein, Yuval
    Bentolila, Laurent A.
    NATURE NANOTECHNOLOGY, 2010, 5 (02) : 99 - 100
  • [12] Single-molecule detection of DNA hybridization
    Singh-Zocchi, M
    Dixit, S
    Ivanov, V
    Zocchi, G
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (13) : 7605 - 7610
  • [13] Single-molecule detection and tracking in plants
    Langhans, Markus
    Meckel, Tobias
    PROTOPLASMA, 2014, 251 (02) : 277 - 291
  • [14] ABC Spotlight on single-molecule detection
    Günter Gauglitz
    Analytical and Bioanalytical Chemistry, 2020, 412 : 7043 - 7045
  • [15] Single-molecule protein interaction detection
    Nature Methods, 2014, 11 (11) : 1091 - 1091
  • [16] Ultrafast single-molecule electrical detection
    Guo, Xuefeng
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 255
  • [17] Nanopores with a spark for single-molecule detection
    Hui Wang
    Daniel Branton
    Nature Biotechnology, 2001, 19 : 622 - 623
  • [18] Nanopores with a spark for single-molecule detection
    Wang, H
    Branton, D
    NATURE BIOTECHNOLOGY, 2001, 19 (07) : 622 - 623
  • [19] Coupled nanopores for single-molecule detection
    Chou, Yung-Chien
    Lin, Chih-Yuan
    Castan, Alice
    Chen, Joshua
    Keneipp, Rachael
    Yasini, Parisa
    Monos, Dimitri
    Drndic, Marija
    NATURE NANOTECHNOLOGY, 2024, 19 (11) : 1686 - 1692
  • [20] New trends in single-molecule bioanalytical detection
    Macchia, Eleonora
    Manoli, Kyriaki
    Di Franco, Cincia
    Scamarcio, Gaetano
    Torsi, Luisa
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2020, 412 (21) : 5005 - 5014
12345