Use of fluorescent europium chelates as labels for detection of microcystin-LR in Taihu Lake, China

被引：5
|
作者：
Niu Weimin [2 ]
He Enqi [2 ]
Wu Qinggang [2 ]
Zhou Weijie [2 ]
Zhang Yi [1 ]
Huang Biao [1 ]
Zhao Xiaolian [3 ]
机构：
[1] Jiangsu Inst Nucl Med, Key Lab Nucl Med, Dept Biotechnol, Wuxi 214063, Peoples R China
[2] Lab Dept Municipal Ctr Dis Control & Prevent, Wuxi 214023, Peoples R China
[3] Jiangsu Suwei Microbiol Res Co Ltd, Wuxi 214063, Peoples R China
来源：
JOURNAL OF RARE EARTHS | 2012年 / 30卷 / 09期
关键词：
microcystin; time-resolved fluoroimmunoassay (TRFIA); water; dynamic detection; dead algae; rare earths; EXPRESSION; TOXINS;
D O I：
10.1016/S1002-0721(12)60158-6
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A method using the time-resolved fluorescence technology to establish a highly sensitive microcystin-LR (MC-LR) indirect competitive immunoassay was proposed in this work. This method was used to monitor the MC-LR level in source water and treated drinking water from Taihu Lake. Algae in the water samples were removed by centrifugation, and the MC-LR level was quantified using this method. Testing results showed that the sensitivity of this method was 0.01 mu g/L, and the dynamic measuring range was from 0.05 to 2 mu g/L. The average recovery was 115%, and the variation (CV) within and between different batches were 7.3% and 9.7%, respectively. Testing results also indicated that this time-resolved fluoroimmunoassay was sensitive and accurate in measuring MC-LR level, especially for quantitative analysis MC-LR level in bulk water.
引用
收藏
页码：941 / 946
页数：6
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