A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers

被引：90

作者：

Ramallo, IA ^{[1
]}

Zacchino, SA ^{[1
]}

Furlan, RLE ^{[1
]}

机构：

[1] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Catedra Farmacognosia, RA-2002 Rosario, Argentina

来源：

PHYTOCHEMICAL ANALYSIS | 2006年 / 17卷 / 01期

关键词：

bioautography; xanthine oxidase; superoxide radical; plant extracts;

D O I：

10.1002/pca.874

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non-enzymatic version of the autographic assay wherein superoxide is chemically generated. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：15 / 19

页数：5

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