A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers

被引：90
|
作者：
Ramallo, IA [1 ]
Zacchino, SA [1 ]
Furlan, RLE [1 ]
机构：
[1] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Catedra Farmacognosia, RA-2002 Rosario, Argentina
来源：
PHYTOCHEMICAL ANALYSIS | 2006年 / 17卷 / 01期
关键词：
bioautography; xanthine oxidase; superoxide radical; plant extracts;
D O I：
10.1002/pca.874
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non-enzymatic version of the autographic assay wherein superoxide is chemically generated. Copyright (c) 2005 John Wiley & Sons, Ltd.
引用
收藏
页码：15 / 19
页数：5
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