Studies on the erythromycin-modified glassy carbon electrode and interaction with DNA

The chemically modified electrode (CME) which was constructed by covalent attaching erythromycin (ERM) to the glassy carbon (GC) surface was investigated in Tris-HCl buffer (pH = 6.0) by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In the potential range of -0.5 similar to 0.4V, CME yields a pair of stable redox wave. It is the carbonyl group of the ERM molecule immobilized on the GC surface that undergoes two electron redox process involving two protons. The interaction of CME with DNA was also studied by DPV. The CME shows the same interaction with DNA as that in the solution. And the result was proved by fluorescence.