Voltammetric studies on the interaction of epinephrine with DNA base on a glassy carbon electrode modified with Au nanoparticles

The interaction of epinephrine (EP) with DNA, including double-strand DNA (dsDNA) and single-strand DNA (ssDNA) were investigated both in Mcllvaine buffer solution and on a glassy carbon electrode (GCE) modified with Au nanoparticles (nanoAu/GCE). In pH 7.0 Mcllvaine buffer solution, EP had a sensitive oxidation current peak at 0.162 V (vs. SCE) on the nanoAu/GCE. Factors influencing the interaction in Mcllvaine buffer solution were optimized, and some electrochemical parameters, such as number of electron transferred, the standard rate constant of the electrode reaction (K (s)) were compared in the presence and absence of DNA. The binding ratio (m) of EP with DNA and the equilibrium constant (beta) were also calculated. EP did not interact with DNA via intercalation mode.