Metabolomic and Transcriptomic Analyses of Escherichia coli for Efficient Fermentation of L-Fucose

被引:18
|
作者
Kim, Jungyeon [1 ]
Cheong, Yu Eun [1 ]
Jung, Inho [1 ,2 ]
Kim, Kyoung Heon [1 ]
机构
[1] Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea
[2] Korea Forestry Promot Inst, Forest Prod Anal & Certificat Div, Seoul 07570, South Korea
来源
MARINE DRUGS | 2019年 / 17卷 / 02期
基金
新加坡国家研究基金会;
关键词
fucose; fermentation; metabolomics; transcriptomics; Escherichia coli; brown macroalgae; SYSTEMS BIOLOGY; CARBON SOURCE; MACROALGAE; BROWN; ADAPTATION; PLATFORM; PATHWAY; GROWTH;
D O I
10.3390/md17020082
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
L-Fucose, one of the major monomeric sugars in brown algae, possesses high potential for use in the large-scale production of bio-based products. Although fucose catabolic pathways have been enzymatically evaluated, the effects of fucose as a carbon source on intracellular metabolism in industrial microorganisms such as Escherichia coli are still not identified. To elucidate the effects of fucose on cellular metabolism and to find clues for efficient conversion of fucose into bio-based products, comparative metabolomic and transcriptomic analyses were performed on E. coli on L-fucose and on D-glucose as a control. When fucose was the carbon source for E. coli, integration of the two omics analyses revealed that excess gluconeogenesis and quorum sensing led to severe depletion of ATP, resulting in accumulation and export of fucose extracellularly. Therefore, metabolic engineering and optimization are needed for E. coil to more efficiently ferment fucose. This is the first multi-omics study investigating the effects of fucose on cellular metabolism in E. coli. These omics data and their biological interpretation could be used to assist metabolic engineering of E. coli producing bio-based products using fucose-containing brown macroalgae.
引用
收藏
页数:14
相关论文
共 50 条
  • [31] Metabolic Engineering of Escherichia coli to Produce 20-Fucosyllactose via Salvage Pathway of Guanosine 5′-Diphosphate (GDP)-L-Fucose
    Chin, Young-Wook
    Seo, Nari
    Kim, Jae-Han
    Seo, Jin-Ho
    BIOTECHNOLOGY AND BIOENGINEERING, 2016, 113 (11) : 2443 - 2452
  • [32] Enzymatic synthesis of l-fucose from L-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme
    Kim, In Jung
    Kim, Do Hyoung
    Nam, Ki Hyun
    Kim, Kyoung Heon
    BIOTECHNOLOGY FOR BIOFUELS, 2019, 12 (01)
  • [33] Enzymatic synthesis of l-fucose from l-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme
    In Jung Kim
    Do Hyoung Kim
    Ki Hyun Nam
    Kyoung Heon Kim
    Biotechnology for Biofuels, 12
  • [34] Engineering Escherichia coli for an efficient aerobic fermentation platform
    Kang, Zhen
    Geng, Yanping
    Xia, Yong Zhen
    Kang, Junhua
    Qi, Qingsheng
    JOURNAL OF BIOTECHNOLOGY, 2009, 144 (01) : 58 - 63
  • [35] Metabolomic and Transcriptomic Analyses of Lycium barbarum L. under Heat Stress
    Qin, Xiaoya
    Qin, Beibei
    He, Wei
    Chen, Yan
    Yin, Yue
    Cao, Youlong
    An, Wei
    Mu, Zixin
    Qin, Ken
    SUSTAINABILITY, 2022, 14 (19)
  • [36] Efficient Biofilm-Based Fermentation Strategies for L-Threonine Production by Escherichia coli
    Chen, Tianpeng
    Liu, Na
    Ren, Peifang
    Xi, Xun
    Yang, Leyun
    Sun, Wenjun
    Yu, Bin
    Ying, Hanjie
    Ouyang, Pingkai
    Liu, Dong
    Chen, Yong
    FRONTIERS IN MICROBIOLOGY, 2019, 10
  • [37] Functional expression of a human GDP-L-fucose transporter in Escherichia coli
    Forster-Fromme, Karin
    Schneider, Sarah
    Sprenger, Georg A.
    Albermann, Christoph
    BIOTECHNOLOGY LETTERS, 2017, 39 (02) : 219 - 226
  • [38] Functional expression of a human GDP-l-fucose transporter in Escherichia coli
    Karin Förster-Fromme
    Sarah Schneider
    Georg A. Sprenger
    Christoph Albermann
    Biotechnology Letters, 2017, 39 : 219 - 226
  • [39] Efficient Biosynthesis of Difucosyllactose via De Novo GDP-l-Fucose Pathway in Metabolically Engineered Escherichia coli
    Liang, Shanquan
    He, Zi
    Liu, Dan
    Yang, Shaoqing
    Yan, Qiaojuan
    Jiang, Zhengqiang
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2024, 72 (08) : 4367 - 4375
  • [40] Transcriptomic and metabolomic investigation of molecular inactivation mechanisms in Escherichia coli triggered by graphene quantum dots
    Fang, Yan
    Wu, Wanfeng
    Zhao, Yan
    Liu, Haoqiang
    Li, Zongda
    Li, Xinbo
    Zhang, Minwei
    Qin, Yanan
    CHEMOSPHERE, 2023, 311