Typing of food-borne Listeria monocytogenes by the optimized repetitive extragenic palindrome-based polymerase chain reaction

被引：0
|
作者：
Pangallo, D [1 ]
Karpísková, R [1 ]
Turna, J [1 ]
Kuchta, T [1 ]
机构：
[1] Inst Food Res, Dept Microbiol & Chem, SK-82475 Bratislava 26, Slovakia
来源：
MICROBIOLOGICA | 2002年 / 25卷 / 04期
关键词：
Listeria monocytogenes; PCR; repetitive extragenic palindrome;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The repetitive extragenic palindrome-based polymerase chain reaction was optimized for typing Listeria monocytogenes by 1) using the QIAamp method to increase the reproducibility of DNA isolation, 2) running PCR with three different DNA concentrations in parallel, 3) using antibody-protected thermostable DNA polymerase to reduce non-specific priming, and 4) using an improved temperature programme to increase the amplification yield. When applied to 42 L. monocytogenes strains isolated from food in the Czech and Slovak republics during 19992000, profiles of 7 - 15 DNA fragments of 330 - 3310 bp were amplified. Based on REP-profiles, strains (serotypes 1/2 and 4) could be divided into 12 groups.
引用
下载
收藏
页码：449 / 454
页数：6
相关论文
共 50 条
  • [1] Typing of food-borne Listeria monocytogenes by polymerase chain reaction-restriction enzyme analysis and amplified fragment length polymorphism
    Mikasová, E
    Oravcová, K
    Kaclíková, T
    Kuchta, T
    Drahovská, H
    NEW MICROBIOLOGICA, 2005, 28 (03): : 265 - 270
  • [2] Fatty aacid typing analysis of food-borne listeria monocytogenes in Hebei area
    Di, Hui-Ling
    Zhang, Yao-Qi
    Shan, Xiao-Xiao
    Yan, He
    Shi, Lei
    Modern Food Science and Technology, 2014, 30 (11) : 65 - 70
  • [3] RAPID DETECTION OF listeria monocytogenes IN FOOD BY POLYMERASE CHAIN REACTION
    Ennaji, H.
    Timinouni, M.
    Ennaji, M. M.
    Ait, M'Hand R.
    Hassar, M.
    Cohen, N.
    CELLULAR AND MOLECULAR BIOLOGY, 2009, 55 : OL1104 - OL1110
  • [4] Advantages of and limits to application of the polymerase chain reaction (PCR) to detection and typing of food-borne pathogenic bacteria
    Lucke, FK
    ten Bosch, C
    DEUTSCHE LEBENSMITTEL-RUNDSCHAU, 1998, 94 (06) : 182 - 187
  • [5] Comparative genotyping of Streptococcus mutans by repetitive extragenic palindromic polymerase chain reaction and multilocus sequence typing
    Momeni, S. S.
    Whiddon, J.
    Moser, S. A.
    Cheon, K.
    Ruby, J. D.
    Childers, N. K.
    MOLECULAR ORAL MICROBIOLOGY, 2013, 28 (01) : 18 - 27
  • [6] Comparison of three polymerase chain reaction-based methods for the detection of Listeria monocytogenes in food
    Kaclíková, E
    Oravcová, K
    Kuchta, T
    Pangallo, D
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2004, 12 (02): : 107 - 113
  • [7] PROBES AND POLYMERASE CHAIN-REACTION FOR DETECTION OF FOOD-BORNE BACTERIAL PATHOGENS
    OLSEN, JE
    AABO, S
    HILL, W
    NOTERMANS, S
    WERNARS, K
    GRANUM, PE
    POPOVIC, T
    RASMUSSEN, HN
    OLSVIK, O
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1995, 28 (01) : 1 - 78
  • [8] USE OF POLYMERASE CHAIN-REACTION FOR DETECTION OF LISTERIA-MONOCYTOGENES IN FOOD
    NIEDERHAUSER, C
    CANDRIAN, U
    HOFELEIN, C
    JERMINI, M
    BUHLER, HP
    LUTHY, J
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (05) : 1564 - 1568
  • [9] IDENTIFICATION OF LISTERIA-MONOCYTOGENES IN FOOD AND ENVIRONMENT BY POLYMERASE CHAIN-REACTION
    SALZANO, G
    PALLOTTA, ML
    MADDONNI, MF
    COPPOLA, R
    VILLANI, F
    SORLINI, C
    JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-ENVIRONMENTAL SCIENCE AND ENGINEERING & TOXIC AND HAZARDOUS SUBSTANCE CONTROL, 1995, 30 (01): : 63 - 71
  • [10] A single space polymerase chain reaction for combined gene detection and epidemiological typing of Listeria monocytogenes
    Medeiros, D
    Farber, JM
    FOOD MICROBIOLOGY, 2001, 18 (04) : 375 - 386
12345