Comparison of Fluorescence Correlation Spectrometry with the Ordinary Fluorescence Optical Configuration with Flow Cytometry as a Tool for Micrometer-level Size Determination

被引：0

作者：

Fujiwara, Kitao ^{[1
]}

Hirokawa, Shun ^{[1
]}

Aoki, Motohide ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Life Sci, Tokyo 1920392, Japan

来源：

ANALYTICAL SCIENCES | 2008年 / 24卷 / 12期

关键词：

D O I：

10.2116/analsci.24.1623

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

As a tool for micrometer-level size determination, fluorescence correlation spectroscopy (FCS), performed based on our previous report, was compared to flow cytometry (FCM). For this purpose, standard fluorescent beads were subjected to both methods. And hence, it was found that our FCS is a useful method with satisfactory precision for size determinations of individual particles at micrometer size levels, while providing the average size for a mixture of two kinds of particles with different sizes.

引用

页码：1623 / 1627

页数：5

共 9 条

[1] Comparison of Fluorescence Correlation Spectrometry with the Ordinary Fluorescence Optical Configuration with Flow Cytometry as a Tool for Micrometer-level Size Determination
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Analytical Sciences, 2008, 24 : 1623 - 1627
[2] Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry
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[7] COUNTING OF SOMATIC-CELLS IN MILK - STUDIES ON THE MEASUREMENT USING FLOW-CYTOMETRY (SOMACOUNT) AND COMPARISON WITH THE MEASURING RESULTS OBTAINED BY MEANS OF THE FLUORESCENCE OPTICAL-SYSTEM (FOSSOMATIC-360)
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[8] A comparison of continuous flow hydride generation laser-induced fluorescence and laser-enhanced ionization spectrometry approaches for parts per trillion level measurements of arsenic, selenium and antimony
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[9] Comparison of matrix-effect corrections for ordinary and uncertainty weighted linear regressions and determination of major element mean concentrations and total uncertainties of sixty-two international geochemical reference materials from wavelength-dispersive X-ray fluorescence spectrometry
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