Structural and Functional Characterization of the Protein Kinase Mps1 in Arabidopsis thaliana

被引:14
|
作者
Gamosa de Oliveira, Eduardo Alves [2 ,3 ]
Romeiro, Nelilma Correia [3 ]
Ribeiro, Elane da Silva [1 ]
Santa-Catarina, Claudete [5 ]
Amancio Oliveira, Antonia Elenir [1 ]
Silveira, Vanildo [4 ]
de Souza Filho, Goncalo Apolinario [4 ]
Venancio, Thiago Motta [1 ]
Lopes Cruz, Marco Antonio [2 ]
机构
[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Quim & Funcao Prot & Peptideos, Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Lab Biotecnol Vegetal, Nucleo Ecol & Desenvolvimento Socioambiental Maca, Rio De Janeiro, Brazil
[3] Univ Fed Rio de Janeiro, Lab Integrado Computacao Cient, Nucleo Ecol & Desenvolvimento Socioambiental Maca, Rio De Janeiro, Brazil
[4] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biotecnol, Rio De Janeiro, Brazil
[5] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biol Celular & Tecidual, Rio De Janeiro, Brazil
来源
PLOS ONE | 2012年 / 7卷 / 09期
关键词
POLE BODY DUPLICATION; CYCLIN-DEPENDENT KINASES; PLANT-CELL CYCLE; SPINDLE-ASSEMBLY CHECKPOINT; LATERAL ROOT INITIATION; MITOTIC CHECKPOINT; PHOSPHORYLATION SITES; GENE-EXPRESSION; SUBCELLULAR-LOCALIZATION; KINETOCHORE LOCALIZATION;
D O I
10.1371/journal.pone.0045707
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In eukaryotes, protein kinases catalyze the transfer of a gamma-phosphate from ATP (or GTP) to specific amino acids in protein targets. In plants, protein kinases have been shown to participate in signaling cascades driving responses to environmental stimuli and developmental processes. Plant meristems are undifferentiated tissues that provide the major source of cells that will form organs throughout development. However, non-dividing specialized cells can also dedifferentiate and re-initiate cell division if exposed to appropriate conditions. Mps1 (Monopolar spindle) is a dual-specificity protein kinase that plays a critical role in monitoring the accuracy of chromosome segregation in the mitotic checkpoint mechanism. Although Mps1 functions have been clearly demonstrated in animals and fungi, its role in plants is so far unclear. Here, using structural and biochemical analyses here we show that Mps1 has highly similar homologs in many plant genomes across distinct lineages (e. g. AtMps1 in Arabidopsis thaliana). Several structural features (i.e. catalytic site, DFG motif and threonine triad) are clearly conserved in plant Mps1 kinases. Structural and sequence analysis also suggest that AtMps1 interact with other cell cycle proteins, such as Mad2 and MAPK1. By using a very specific Mps1 inhibitor (SP600125) we show that compromised AtMps1 activity hampers the development of A. thaliana seedlings in a dose-dependent manner, especially in secondary roots. Moreover, concomitant administration of the auxin IAA neutralizes the AtMps1 inhibition phenotype, allowing secondary root development. These observations let us to hypothesize that AtMps1 might be a downstream regulator of IAA signaling in the formation of secondary roots. Our results indicate that Mps1 might be a universal component of the Spindle Assembly Checkpoint machinery across very distant lineages of eukaryotes.
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页数:9
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