Continuous-flow/stopped-flow system incorporating a rotating bioreactor for the determination of penicillins in pharmaceutical products

被引：5
|
作者：
Lapierre, AV [1 ]
Olsina, RA [1 ]
Raba, J [1 ]
机构：
[1] Natl Univ San Luis, Dept Analyt Chem, San Luis, Argentina
来源：
ANALYTICA CHIMICA ACTA | 1999年 / 396卷 / 2-3期
关键词：
bioreactor; penicillins; penicillinase; flow system biosensors;
D O I：
10.1016/S0003-2670(99)00445-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A rotating enzyme-immobilized reactor and a flat pH electrode have been incorporated into a sealed cell for use under continuous-flow/stopped-flow operation. The system has been used for rapid determination of penicillins G and V in pharmaceutical tablets and injectables. Detections based on the increase of hydrogen ion concentration due to enzymatic hydrolysis of the penicillins by the enzyme penicillinase [EC 3.5.2.6] immobilized on controlled-pore glass. Selective determination of the analyte in the 0.001-0.50 mM concentration range can be accomplished at the rate of 30 samples h(-1). (C)1999 Elsevier Science B.V. All rights reserved.
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页码：143 / 149
页数：7
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