Polyester Cloth-Based Hybridization Array System for Identification of Enterohemorrhagic Escherichia coli Serogroups O26, O45, O103, O111, O121, O145, and O157

被引：21
|
作者：
Blais, Burton W. [1 ]
Gauthier, Martine [1 ]
Deschenes, Mylene [1 ]
Huszczynski, George [1 ]
机构：
[1] Canadian Food Inspect Agcy, Ontario Lab Network, Ottawa, ON K1A 0C6, Canada
来源：
JOURNAL OF FOOD PROTECTION | 2012年 / 75卷 / 09期
关键词：
PCR ASSAYS;
D O I：
10.4315/0362-028X.JFP-12-116
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A cloth-based hybridization array system (CHAS) was developed for the identification of foodborne colony isolates of seven priority enterohemorrhagic Escherichia coli (EHEC-7) serogroups targeted by U.S. food inspection programs. Gene sequences associated with intimin; Shiga-like toxins 1 and 2; and the antigenic markers O26, O45, O103, O111, O121, O145, and O157 were amplified in a multiplex PCR incorporating a digoxigenin label, and detected by hybridization of the PCR products with an array of specific oligonucleotide probes immobilized on a polyester cloth support, with subsequent immunoenzymatic assay of the captured amplicons. The EHEC-7 CHAS exhibited 100% inclusivity and 100% exclusivity characteristics with respect to detection of the various markers among 89 different E. coli strains, with various marker gene profiles and 15 different strains of non-E. coli bacteria.
引用
收藏
页码：1691 / 1697
页数：7
相关论文
共 50 条
  • [41] Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111
    Eichhorn, Inga
    Semmler, Torsten
    Mellmann, Alexander
    Pickard, Derek
    Anjum, Muna F.
    Fruth, Angelika
    Karch, Helge
    Wieler, Lothar H.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2018, 308 (08) : 1085 - 1095
  • [42] Novel differential and confirmation plating media for Shiga toxin-producing Escherichia coli serotypes O26, O103, O111, O145 and sorbitol-positive and -negative O157
    Posse, Bjoern
    De Zutter, Lieven
    Heyndrickx, Marc
    Herman, Lieve
    FEMS MICROBIOLOGY LETTERS, 2008, 282 (01) : 124 - 131
  • [43] Salmonella and Enterohemorrhagic Escherichia coli Serogroups O45, O121,O145 in Wheat Flour: Effects of Long-Term Storage and Thermal Treatments
    Forghani, Fereidoun
    den Bakker, Meghan
    Liao, Jye-Yin
    Payton, Alison S.
    Futral, Alexandra N.
    Diez-Gonzalez, Francisco
    FRONTIERS IN MICROBIOLOGY, 2019, 10
  • [44] A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables
    Tzschoppe, Markus
    Martin, Annett
    Beutin, Lothar
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2012, 152 (1-2) : 19 - 30
  • [45] Screening food raw materials for the presence of the world's most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157
    Perelle, Sylvie
    Dilasser, Francoise
    Grout, Joel
    Fach, Patrick
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 113 (03) : 284 - 288
  • [46] Genetic characteristics of Shiga toxin-producing E. coli O157, O26, O103, O111 and O145 isolates from humans, food, and cattle in Belgium
    Verstraete, K.
    De Reu, K.
    Van Weyenberg, S.
    Pierard, D.
    De Zutter, L.
    Herman, L.
    Robyn, J.
    Heyndrickx, M.
    EPIDEMIOLOGY AND INFECTION, 2013, 141 (12): : 2503 - 2515
  • [47] Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables
    Safaríková, M
    Safarík, I
    LETTERS IN APPLIED MICROBIOLOGY, 2001, 33 (01) : 36 - 39
  • [48] Antimicrobial resistance of Escherichia coli O26, O103, O111, O128, and O145 from animals and humans (vol 8, pg 1409, 2002)
    Schroeder, CM
    EMERGING INFECTIOUS DISEASES, 2003, 9 (01) : 140 - 140
  • [49] Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo
    Girard, Francis
    Dziva, Francis
    van Diemen, Pauline
    Phillips, Alan D.
    Stevens, Mark P.
    Frankel, Gad
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (09) : 3084 - 3090
  • [50] Effect of the enrichment time and immunomagnetic separation on the detection of Shiga toxin-producing Escherichia coli O26, O103, O111, O145 and sorbitol positive O157 from artificially inoculated cattle faeces
    Verstraete, Karen
    De Zutter, Lieven
    Messens, Winy
    Herman, Lieve
    Heyndrickx, Marc
    De Reu, Koen
    VETERINARY MICROBIOLOGY, 2010, 145 (1-2) : 106 - 112
12345