Polyester Cloth-Based Hybridization Array System for Identification of Enterohemorrhagic Escherichia coli Serogroups O26, O45, O103, O111, O121, O145, and O157

被引：21
|
作者：
Blais, Burton W. [1 ]
Gauthier, Martine [1 ]
Deschenes, Mylene [1 ]
Huszczynski, George [1 ]
机构：
[1] Canadian Food Inspect Agcy, Ontario Lab Network, Ottawa, ON K1A 0C6, Canada
来源：
JOURNAL OF FOOD PROTECTION | 2012年 / 75卷 / 09期
关键词：
PCR ASSAYS;
D O I：
10.4315/0362-028X.JFP-12-116
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A cloth-based hybridization array system (CHAS) was developed for the identification of foodborne colony isolates of seven priority enterohemorrhagic Escherichia coli (EHEC-7) serogroups targeted by U.S. food inspection programs. Gene sequences associated with intimin; Shiga-like toxins 1 and 2; and the antigenic markers O26, O45, O103, O111, O121, O145, and O157 were amplified in a multiplex PCR incorporating a digoxigenin label, and detected by hybridization of the PCR products with an array of specific oligonucleotide probes immobilized on a polyester cloth support, with subsequent immunoenzymatic assay of the captured amplicons. The EHEC-7 CHAS exhibited 100% inclusivity and 100% exclusivity characteristics with respect to detection of the various markers among 89 different E. coli strains, with various marker gene profiles and 15 different strains of non-E. coli bacteria.
引用
收藏
页码：1691 / 1697
页数：7
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