Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

被引：19

作者：

Nkere, Chukwuemeka K. ^{[1
,2
,3
]}

Oyekanmi, Joshua O. ^{[1
]}

Silva, Goncalo ^{[4
]}

Bomer, Moritz ^{[4
]}

Atiri, Gabriel I. ^{[2
]}

Onyeka, Joseph ^{[3
]}

Maroya, Norbert G. ^{[1
]}

Seal, Susan E. ^{[4
]}

Kumar, P. Lava ^{[1
]}

机构：

[1] IITA, Oyo Rd,PMB 5320, Ibadan, Nigeria

[2] Univ Ibadan, Dept Crop Protect & Environm Biol, Ibadan, Nigeria

[3] Natl Root Crops Res Inst, Umudike, Nigeria

[4] Univ Greenwich, Nat Resources Inst, Chatham ME4 4TB, Kent, England

来源：

ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 04期

基金：

比尔及梅琳达.盖茨基金会;

关键词：

RECOMBINASE POLYMERASE AMPLIFICATION; PCR; ASSAY;

D O I：

10.1007/s00705-018-3706-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.

引用

页码：1057 / 1061

页数：5

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