Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

被引：19
|
作者：
Nkere, Chukwuemeka K. [1 ,2 ,3 ]
Oyekanmi, Joshua O. [1 ]
Silva, Goncalo [4 ]
Bomer, Moritz [4 ]
Atiri, Gabriel I. [2 ]
Onyeka, Joseph [3 ]
Maroya, Norbert G. [1 ]
Seal, Susan E. [4 ]
Kumar, P. Lava [1 ]
机构：
[1] IITA, Oyo Rd,PMB 5320, Ibadan, Nigeria
[2] Univ Ibadan, Dept Crop Protect & Environm Biol, Ibadan, Nigeria
[3] Natl Root Crops Res Inst, Umudike, Nigeria
[4] Univ Greenwich, Nat Resources Inst, Chatham ME4 4TB, Kent, England
来源：
ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 04期
基金：
比尔及梅琳达.盖茨基金会;
关键词：
RECOMBINASE POLYMERASE AMPLIFICATION; PCR; ASSAY;
D O I：
10.1007/s00705-018-3706-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.
引用
收藏
页码：1057 / 1061
页数：5
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