Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates

被引：37
|
作者：
Rodriguez-Sanchez, Belen [1 ,2 ,3 ]
Jesus Ruiz-Serrano, Mara [1 ,2 ,3 ]
Marin, Mercedes [1 ,2 ,3 ,4 ]
Lopez Roa, Paula [1 ]
Rodriguez-Creixems, Marta [1 ]
Bouza, Emilio [1 ,2 ,3 ,4 ]
机构：
[1] Univ Gregorio Maranon, Gen Hosp, Clin Microbiol & Infect Dis Dept, Madrid, Spain
[2] CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain
[3] Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
[4] Univ Complutense Madrid, Sch Med, Dept Med, Madrid, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 08期
关键词：
RAPID IDENTIFICATION; CULTURE-MEDIA;
D O I：
10.1128/JCM.01380-15
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of nontuberculous mycobacterial (NTM) isolates was evaluated in this study. Overall, 125 NTM isolates were analyzed by MALDI-TOF and GenoType CM/AS. Identification by 16S rRNA/hsp65 sequencing was considered the gold standard. Agreements between MALDI-TOF and GenoType CM/AS with the reference method were, respectively, 94.4% and 84.0%. In 17 cases (13.6%), results provided by GenoType and MALDI-TOF were discordant; however, the reference method agreed with MALDI-TOF in 16/17 cases (94.1%; P = 0.002).
引用
收藏
页码：2737 / 2740
页数：4
相关论文
共 50 条
  • [41] Identification of a Variety of Staphylococcus Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
    Dubois, Damien
    Leyssene, David
    Chacornac, Jean Paul
    Kostrzewa, Markus
    Schmit, Pierre Olivier
    Talon, Regine
    Bonnet, Richard
    Delmas, Julien
    JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (03) : 941 - 945
  • [42] Update on Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Identification of Filamentous Fungi
    Wilkendorf, Laura S.
    Bowles, Edmee
    Buil, Jochem B.
    van der Lee, Henrich A. L.
    Posteraro, Brunella
    Sanguinetti, Maurizio
    Verweij, Paul E.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2020, 58 (12)
  • [43] Identification and Typing of Lactococcus lactis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
    Tanigawa, Kana
    Kawabata, Hiroaki
    Watanabe, Koichi
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (12) : 4055 - 4062
  • [44] Accurate identification of Escherichia albertii by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Hatanaka, Noritoshi
    Awasthi, Sharda Prasad
    Hinenoya, Atsushi
    Ueda, Osamu
    Yamasaki, Shinji
    JOURNAL OF MICROBIOLOGICAL METHODS, 2020, 173
  • [45] Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Mani, Rinosh
    Vilela, Raquel
    Kettler, Niesa
    Chilvers, Martin I.
    Mendoza, Leonel
    JOURNAL OF MEDICAL MICROBIOLOGY, 2019, 68 (04) : 574 - 584
  • [46] Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Identification of Mycobacteria in Routine Clinical Practice
    El Khechine, Amel
    Couderc, Carine
    Flaudrops, Christophe
    Raoult, Didier
    Drancourt, Michel
    PLOS ONE, 2011, 6 (09):
  • [47] Identification of Haemophilus influenzae Type b Isolates by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
    Mansson, Viktor
    Resman, Fredrik
    Kostrzewa, Markus
    Nilson, Bo
    Riesbeck, Kristian
    JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (07) : 2215 - 2224
  • [48] Successful Identification of Clinical Dermatophyte and Neoscytalidium Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
    Alshawa, Kinda
    Beretti, Jean-Luc
    Lacroix, Claire
    Feuilhade, Martine
    Dauphin, Brunhilde
    Quesne, Gilles
    Hassouni, Noura
    Nassif, Xavier
    Bougnoux, Marie-Elisabeth
    JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (07) : 2277 - 2281
  • [49] Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of human oral Capnocytophaga species
    Jolivet-Gougeon, Anne
    Helsens, Nicolas
    Renard, Elise
    Tamanai-Shacoori, Zohreh
    Bonnaure-Mallet, Martine
    ANAEROBE, 2017, 48 : 89 - 93
  • [50] Restricted Identification of Clinical Pathogens Categorized as Biothreats by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
    Keller, Peter M.
    Bruderer, Vera
    Mueller, Franco
    JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (03) : 816 - 816
12345