Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates

被引：37
|
作者：
Rodriguez-Sanchez, Belen [1 ,2 ,3 ]
Jesus Ruiz-Serrano, Mara [1 ,2 ,3 ]
Marin, Mercedes [1 ,2 ,3 ,4 ]
Lopez Roa, Paula [1 ]
Rodriguez-Creixems, Marta [1 ]
Bouza, Emilio [1 ,2 ,3 ,4 ]
机构：
[1] Univ Gregorio Maranon, Gen Hosp, Clin Microbiol & Infect Dis Dept, Madrid, Spain
[2] CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain
[3] Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
[4] Univ Complutense Madrid, Sch Med, Dept Med, Madrid, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 08期
关键词：
RAPID IDENTIFICATION; CULTURE-MEDIA;
D O I：
10.1128/JCM.01380-15
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of nontuberculous mycobacterial (NTM) isolates was evaluated in this study. Overall, 125 NTM isolates were analyzed by MALDI-TOF and GenoType CM/AS. Identification by 16S rRNA/hsp65 sequencing was considered the gold standard. Agreements between MALDI-TOF and GenoType CM/AS with the reference method were, respectively, 94.4% and 84.0%. In 17 cases (13.6%), results provided by GenoType and MALDI-TOF were discordant; however, the reference method agreed with MALDI-TOF in 16/17 cases (94.1%; P = 0.002).
引用
收藏
页码：2737 / 2740
页数：4
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