Expression of recombinant human tumor necrosis factor-α in a baculovirus expression system

被引：2
|
作者：
Zhang, XW [1 ]
Sun, T [1 ]
Zeng, XY [1 ]
Liu, X [1 ]
Gu, DX [1 ]
机构：
[1] Zhongshan Univ, Food Engn Res Ctr, State Educ Commiss, Guangzhou 510275, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 1999年 / 70卷 / 03期
关键词：
TNF-alpha; baculovirus system; transfer vector; gene expression;
D O I：
10.1016/S0960-8524(99)00032-2
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A cDNA fragment coding human tumor necrosis factor-alpha (TNF-alpha) was inserted into the vector pSXIVVI(+)X3 with the control of Syn XIV promoter. The Sf9 cells (Spodoptera frugiperda) were co-transfected with the recombinant plasmid and TnNPV DNA (Trichoplusia ni nuclear polyhedrosis virus DNA). Cells infected with recombinant virus synthesized TNF-alpha protein at a level of about 38% of total cellular protein. TNF-alpha activity in infected cells was measured by L929 cytotoxic assay, the highest expression level, 1.5 x 10(4) U/10(6) cells, was obtained at 76 h after infection. Western blot analysis of protein extracts from infected larvae showed that the virus-mediated TNF-alpha had immunoreactivity. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：299 / 301
页数：3
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