Expression of human stem cell factor in the baculovirus expression system

被引：0
|
作者：
Wang, L
Qin, JC
Shen, BF
Zhu, J
Zang, YH
机构：
[1] NANJING UNIV,DEPT BIOCHEM,NANJING 210093,PEOPLES R CHINA
[2] NANJING UNIV,NATL LAB PHARMACEUT BIOTECHNOL,NANJING 210093,PEOPLES R CHINA
[3] NATL INST MIL MED SCI,BEIJING 100850,PEOPLES R CHINA
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 37卷 / 04期
关键词：
stem cell factor; baculovirus expression system;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A partial cDNA of human Stem Cell Factor (SCF) was isolated and transferred into the genome of Bombyx Mori Nuclear Polyhedrosis Virus adjacent to the polyhedrin promoter. The soluble form of the SCF protein was expressed in Bombyx Mori cells and silkworm larvae and was secreted into the medium in an active state. The recombinant SCF was similar to natural SCF with respect to its interaction with monoclonal antibody and its synergistic biological activity in conjunction with colony-stimulating factor.
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页码：729 / 736
页数：8
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