Shewanella inventionis sp nov., isolated from deep-sea sediment

A novel Gram-stain-negative, rod-shaped bacterial strain, designated KX27(T), was isolated from deep-sea sediment of the Okinawa Trough and characterized on the basis of a polyphasic taxonomic approach. The phenotypic features of strain KX27(T) were similar to those of members of the genus Shewanella. Strain KX27(T) exhibited highest 16S rRNA gene sequence similarity (98.37%) to Shewanefla basaltis JCM 14937(T). Levels of gyrB gene sequence similarity between strain KX27(T) and its closest genetic neighbours were lower than 90%, the species cut-off value for Shewanefla. The DNA DNA hybridization value between strain KX27(T) and S. basaltis JCM 14937(T) (54%) was lower than the accepted species threshold. The predominant cellular fatty acids of strain KX27(T) were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-17:1 omega 8c, summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6C) and iso-C-15:0. The major polar lipids of strain KX27(T) were phosphatidylethanolamine and phosphatidylglycerol. On the basis of combined phenotypic, chemotaxonomic and phylogenetic analysis, strain KX27(T) represents a novel species of the genus Shewanella, for which the name Shewanefla inventionis sp. nov. is proposed. The type strain is KX27(T) (=KCTC 42807(T)=CGMCC 1.15339(T)).