Salinimicrobium sediminis sp nov., isolated from a deep-sea sediment

Strain JC207(T) was isolated from a deep (265 m) sea sediment, and appeared as dark yellow colonies on agar plates with cells staining Gram-negative. Catalase, oxidase and caseinase were positive, while chitinase, gelatinase and amylase were negative. Major (>5%) fatty acids were iso-C-15:0, anteiso-C-15:0, iso-C-17:1 omega 9c, iso-C-16:0, iso-C-15:0 3-OH, iso-C-17:0 3-OH, iso-C-14:0 and iso-C(15:1)G. Strain JC207(T) contained phosphatidylethanolamine as the major polar lipid, with minor amounts of five unidentified lipids. A bacterial hopane derivative, diplopterol and adenosylhopane were the major hopanoids. Genomic DNA G+C content was 47.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC207(T) represented a member of the genus Salinimicrobium within the family Flavobacteriaceae of the phylum Bacteroidetes. Strain JC207(T). had sequence similarity with Salinimicrobium terrae YIM-C338(T). (98 %), Salinimicrobium xinjiangense BH206(T) (97.6%) and other members of the genus Salinimicrobium (<96.8%). However, strain JC207(T) showed an average of 23.6 +/- 4 and 37 +/- 4 relatedness (based on DNA-DNA hybridization) with Salinimicrobium terrae CGMCC 1.6308(T) (=YIM-C338(T)) and Salinimicrobium xinjiangense KCTC 12883(T) (=BH206(T)), respectively. Morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC207(T) as a representative of a novel species in the genus Salinimicrobium, for which the name Salinimicrobium sediminis sp. nov. is proposed. The type strain is JC207(T) (=KCTC 32444(T)=CGMCC 1.12641(T)).