An electrochemical DNA sensor for detection of cytokeratin 19

被引：3

作者：

Xu, Xiongwei ^{[1
]}

Weng, Xiuhua ^{[1
,2
]}

Liu, Ailin ^{[2
]}

Zhang, Xiaoxing ^{[2
]}

Wang, Changlian ^{[1
]}

Chen, Wei ^{[2
]}

Lin, Xinhua ^{[2
]}

机构：

[1] Fujian Med Univ, Affiliated Hosp 1, Dept Pharm, Fuzhou 350005, Peoples R China

[2] Fujian Med Univ, Dept Pharmaceut Anal, Fuzhou 350004, Peoples R China

来源：

ANALYTICAL METHODS | 2013年 / 5卷 / 09期

基金：

中国国家自然科学基金;

关键词：

PROBE CARRIER PLATFORM; HAIRPIN LNA PROBE; NUCLEIC-ACIDS; HYBRIDIZATION; BIOSENSOR; NANOPARTICLES; RECOGNITION; SURFACE; INDICATOR; ELECTRODE;

D O I：

10.1039/c3ay00037k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

33258 as the hybridization indicator was reported. The biosensor was developed by using thiolated ssDNA as the capture probe for hybridization with CK19 gene on a gold electrode. A significant increase of the peak current for Hoechst 33258 upon hybridization of immobilized ssDNA with target DNA was observed. This peak current change was used to monitor the recognition of the CK19 DNA sequence. Immobilization and hybridization conditions determining the performance of the electrochemical assay were investigated. Under optimal conditions, the constructed electrochemical DNA biosensor has excellent specificity for single-base mismatch and complementary after hybridization. A detection limit of 1.8 x 10(-8) M for complementary target DNA can be

引用

页码：2329 / 2334

页数：6

共 50 条

[41] Electrochemical DNA sensors for detection of DNA damage
Diculescu, VC
Paquim, AMC
Brett, AMO
SENSORS, 2005, 5 (6-10) : 377 - 393
[42] Electrochemical switching with a DNA aptamer-based electrochemical sensor
Beiranvand, Shabnam
Azadbakht, Azadeh
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2017, 76 : 925 - 933
[43] ELECTROCHEMICAL SENSOR SYSTEM FOR GLYPHOSATE DETECTION
Uka, Besnik
Kieninger, Jochen
Urban, Gerald A.
Weltin, Andreas
2021 21ST INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS), 2021, : 791 - 794
[44] A disposable electrochemical sensor for vanillin detection
Bettazzi, F
Palchetti, I
Sisalli, S
Mascini, M
ANALYTICA CHIMICA ACTA, 2006, 555 (01) : 134 - 138
[45] Electrochemical Sensor for Multiplex Biomarkers Detection
Wei, Fang
Patel, Prabhudas
Liao, Wei
Chaudhry, Kishore
Zhang, Lei
Arellano-Garcia, Martha
Hu, Shen
Elashoff, David
Zhou, Hui
Shukla, Shilin
Shah, Franky
Ho, Chih-Ming
Wong, David T.
CLINICAL CANCER RESEARCH, 2009, 15 (13) : 4446 - 4452
[46] Electrochemical sensor for homemade explosives detection
Lu, Donglai
Wang, Joseph
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2009, 237 : 194 - 194
[47] Electrochemical Sensor for the Detection of Lead Ions
Anambig, I. V.
Suganthan, V.
Raj, N. Arunai Nambi
Kumar, A. Siva
2013 INTERNATIONAL CONFERENCE ON ADVANCED NANOMATERIALS AND EMERGING ENGINEERING TECHNOLOGIES (ICANMEET), 2013, : 367 - 370
[48] Detection EGFR exon 19 status of lung cancer patients by DNA electrochemical biosensor
Xu, Xiong-Wei
Weng, Xiu-Hua
Wang, Chang-Lian
Lin, Wei -Wei
Liu, Ai-Lin
Chen, Wei
Lin, Xin-Hua
BIOSENSORS & BIOELECTRONICS, 2016, 80 : 411 - 417
[49] An electrochemical DNA sensor based on an integrated and automated DNA walker
Fan, Hao
Wu, Ying
Huang, Tongfu
Hong, Nian
Cui, Hanfeng
Wei, Guobing
Liao, Fusheng
Zhang, Jing
BIOELECTROCHEMISTRY, 2022, 147
[50] Electrochemical DNA sensor for anthrax toxin activator gene atxA-detection of PCR amplicons
Das, Ritu
Goel, Ajay K.
Sharma, Mukesh K.
Upadhyay, Sanjay
BIOSENSORS & BIOELECTRONICS, 2015, 74 : 939 - 946

← 1 2 3 4 5 →