An electrochemical DNA sensor for detection of cytokeratin 19

被引：3

作者：

Xu, Xiongwei ^{[1
]}

Weng, Xiuhua ^{[1
,2
]}

Liu, Ailin ^{[2
]}

Zhang, Xiaoxing ^{[2
]}

Wang, Changlian ^{[1
]}

Chen, Wei ^{[2
]}

Lin, Xinhua ^{[2
]}

机构：

[1] Fujian Med Univ, Affiliated Hosp 1, Dept Pharm, Fuzhou 350005, Peoples R China

[2] Fujian Med Univ, Dept Pharmaceut Anal, Fuzhou 350004, Peoples R China

来源：

ANALYTICAL METHODS | 2013年 / 5卷 / 09期

基金：

中国国家自然科学基金;

关键词：

PROBE CARRIER PLATFORM; HAIRPIN LNA PROBE; NUCLEIC-ACIDS; HYBRIDIZATION; BIOSENSOR; NANOPARTICLES; RECOGNITION; SURFACE; INDICATOR; ELECTRODE;

D O I：

10.1039/c3ay00037k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

33258 as the hybridization indicator was reported. The biosensor was developed by using thiolated ssDNA as the capture probe for hybridization with CK19 gene on a gold electrode. A significant increase of the peak current for Hoechst 33258 upon hybridization of immobilized ssDNA with target DNA was observed. This peak current change was used to monitor the recognition of the CK19 DNA sequence. Immobilization and hybridization conditions determining the performance of the electrochemical assay were investigated. Under optimal conditions, the constructed electrochemical DNA biosensor has excellent specificity for single-base mismatch and complementary after hybridization. A detection limit of 1.8 x 10(-8) M for complementary target DNA can be

引用

页码：2329 / 2334

页数：6

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